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Showing: 101 - 120 of 8946 results

  4. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  5. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid
    #111147
    Purpose
    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.
    Depositor
    Mohan Balasubramanian
    Article
    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827
    Insert
    actin gamma 1 (ACTG1 Human)
    Use
    Pichia pastoris integration vector
    Tags
    Thymosin beta and a His-tag
    Expression
    Yeast
    Mutation
    codon-optimised for expression in Pichia pastoris
    Promoter
    AOX1
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    111147-plasmid-map-sequence-id-214068

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  6. pJK057

    Plasmid
    #75036
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes residues 2-7 to His (SSRNNP>HHHHHH)
    Promoter
    T7 and n/a
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    75036-plasmid-map-sequence-id-142299

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  7. pEG03

    Plasmid
    #154138
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154138-plasmid-map-sequence-id-332116

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  8. pKSCTMF

    Plasmid
    #85073
    Purpose
    Gene insertion/deletion in Pichia pastoris
    Depositor
    Sheng Yang
    Article
    Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi
    Insert
    AOX1promoter - mazF - AOX1 tt- Zeocin R gene
    Use
    Gene insertion/deletion in pichia pastoris
    Tags
    Expression
    Mutation
    Promoter
    pAOX1
    Available since
    March 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    85073-plasmid-map-sequence-id-174101

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  9. pEG03'

    Plasmid
    #162578
    Purpose
    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_gyrA
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    162578-plasmid-map-sequence-id-332111

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  10. pEG01

    Plasmid
    #154136
    Purpose
    This plasmid carries fucO and aldA under the control of P_BAD promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_BAD
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154136-plasmid-map-sequence-id-332112

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  11. pEG02

    Plasmid
    #154137
    Purpose
    This plasmid carries fucO and aldA under the control of P_thrC3 promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.
    Depositor
    Kang Zhou
    Article
    Panda et al Biochemical Engineering Journal
    Insert
    fucO, aldA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P_thrC3
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154137-plasmid-map-sequence-id-332114

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  14. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  15. pNC2

    Plasmid
    #72438
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Meyer et al Biochemistry. 1999 Mar 9;38(10):3012-8.
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg
    Promoter
    T7 and n/a
    Available since
    May 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72438-plasmid-map-sequence-id-140433

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  18. pCapV

    Plasmid
    #183028
    Purpose
    the patatin-like phosphodiesterase CapV from the animal commensal strain Escherichia coli ECOR31 cloned in the L-arabinose inducible vector pBAD28
    Depositor
    Ute Romling
    Article
    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(
    Insert
    patatin-like phospholipase CapV
    Use
    Tags
    No
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD promoter
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183028-plasmid-map-sequence-id-361615

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Showing: 101 - 120 of 8946 results