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GalT-RpHLuorin2
Plasmid#171719PurposeTrans-Golgi expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)
UseTagsratiometric pHluorin2ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GAL9
Plasmid#166689PurposeFor creating a stable mCherry-GAL9 cell lineDepositorInsertLGALS9 (LGALS9 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorInsertLGALS3 (LGALS3 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGal3
Plasmid#73080PurposeExpression in mammalian cellsDepositorInsertGalectin-3 (LGALS3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-Gal80ts
Plasmid#51619Purposevertebrate expression of full-length temperature-sensitive Gal80 with stopDepositorInsertGal80ts (GAL80 Budding Yeast)
UseVertebrate expressionTagsExpressionMutationtemperature sensitive (G203V, G323R)*PromoterAvailable sinceMarch 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
mAG-GAL3
Plasmid#62734PurposeMammalian vesicle leakage reporterDepositorInsertmAG-Galectin 3
UseLentiviralTagsmAzami-GreenExpressionMammalianMutationPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pBPGAL4.2::VP16Uw
Plasmid#26228DepositorInsertGAL4::VP16 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
Galphai2-mTurquoise2
Plasmid#69621PurposeThe heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2DepositorInsertGalphai2-mTurquoise2
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP
Plasmid#85427PurposeLentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-10
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_T2A_PDL1_PGK_mCherry
Plasmid#85430Purpose[Edit] Lentiviral vector for Gal4 inducible human IL-10/PD-L1 with constitutive mCherry expressionDepositorInserthuman IL-10 T2A PD-L1
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GAL4
Plasmid#24345DepositorInsertGAL4
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP
Plasmid#85428PurposeLentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-12
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
piRFP670-N1-GalT
Plasmid#87325PurposeTo express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferaseDepositorInsert1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
UseTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
GI-Gal4 DBD
Plasmid#42500PurposeGIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factorDepositorInsertGIGANTEA fused with Gal4 DBD
UseTagsGal4 DNA binding domain (DBD)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only
