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Plasmid#103063PurposeExpresses colicin E1 and E1 immunity protein without E1 lysis (kil) geneDepositorInsertColicin E1 and E1 immunity protein
UseTagsExpressionBacterialMutationPromotercolicin promotor from ColE1Available sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_L7Ae-VP64
Plasmid#68421PurposeTransient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoterDepositorInsertL7Ae
UseSynthetic BiologyTagsHA and VP64ExpressionMammalianMutationPromoterhuman UbCAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUBC_V5_MS2-mCherry
Plasmid#68420PurposeTransient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoterDepositorInsertMS2
UseSynthetic BiologyTagsV5 and mCherryExpressionMammalianMutationMS2: V75E/A81GPromoterhuman UbCAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
rbcLS-pMAL-2px
Plasmid#41621DepositorInsertrbcL/rbcS
UseTagsExpressionBacterialMutationPromoterPtacAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-eIF4B
Plasmid#37226DepositorInsertTIF3
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092694
Plasmid#153907PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stopDepositorInsertfragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationwtPromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4092709
Plasmid#153859PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;handful SNPs as watermarks…PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII
Plasmid#80860PurposePhagemid (encodes evolved cI5C6A,P and M13 gene III)DepositorInsertsM13 gene III
cI5C6A,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-PAB1
Plasmid#37234DepositorInsertPAB1 (PAB1 Budding Yeast)
UseTagsinteinExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G,P-J23106-geneIII
Plasmid#80861PurposePhagemid (encodes evolved cI5G6G,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6G,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGJJ055
Plasmid#183622PurposeddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G,P-J23106-geneIII
Plasmid#80864PurposePhagemid (encodes evolved cI4A5C6G7G,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G,P
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-eIF4A
Plasmid#37225DepositorInserteIF4A
UseTagsinteinExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII
Plasmid#80862PurposePhagemid (encodes evolved cI5G6T,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NSm-GFP pZK538
Plasmid#118012Purposeexpresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).DepositorArticleInsertsNSm
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5C6G7G-J23106-geneIII
Plasmid#80909PurposePhagemid (encodes cI4A5C6G7G and M13 gene III)DepositorInsertsM13 gene III
cI4A5C6G7G
UseTagsExpressionBacterialMutation238G to 238D (please see depositor comment below)PromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTYB2-HCR1
Plasmid#37233DepositorInsertHRC1 (HCR1 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
JD134
Plasmid#229490PurposeAdenoviral E4 geneDepositorInsertE4
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterwt promoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only