We narrowed to 5,006 results for: puc

1. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57kan-T7-gRNA-U6 V2

   Plasmid

   #115520

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   sgRNA scaffolf-U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/mChe-C

   Plasmid

   #183160

   Purpose

   Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19/eCFP-C

   Plasmid

   #183156

   Purpose

   Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19_CoV-2_linker

   Plasmid

   #211731

   Purpose

   Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

   Depositor

   Thomas Klimkait

   Article

   Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

   Insert

   Linker fragment SARS-CoV-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-Curlcake3

   Plasmid

   #139342

   Purpose

   Expresses Curlcake3 (CC3) in E.coli

   Depositor

   Eva Maria Novoa

   Article

   Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

   Insert

   Curlcake3 with T7 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-Curlcake1

   Plasmid

   #139340

   Purpose

   Expresses Curlcake1 (CC1) in E.coli

   Depositor

   Eva Maria Novoa

   Article

   Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

   Insert

   Curlcake1 with T7 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-Curlcake2

   Plasmid

   #139341

   Purpose

   Expresses Curlcake2 (CC2) in E.coli

   Depositor

   Eva Maria Novoa

   Article

   Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

   Insert

   Curlcake2 with T7 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-Curlcake4

   Plasmid

   #139343

   Purpose

   Expresses Curlcake4 (CC4) in E.coli

   Depositor

   Eva Maria Novoa

   Article

   Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

   Insert

   Curlcake4 with T7 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57 (Gent)

    Plasmid

    #54338

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/human IL-8

    Plasmid

    #17610

    Depositor

    Joost Oppenheim

    Article

    Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

    Insert

    IL8 (CXCL8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-gRNA

    Plasmid

    #137776

    Purpose

    Contains the T7 promoter and gRNA scaffold and is used for the in vitro transcription of gRNAs

    Depositor

    Kabin Xie

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    pUC19 BsaI destroyed

    Promoter

    T7

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19/mChe-Px

    Plasmid

    #183170

    Purpose

    Construct for transient expression of peroxisomes-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Px
    

    Use

    Tags

    10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/Pd-mChe

    Plasmid

    #183172

    Purpose

    Construct for transient expression of plasmodesmata-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Pd-mCherry
    

    Use

    Tags

    AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-GFP-HS

    Plasmid

    #133305

    Purpose

    "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

    Depositor

    Chris Barnes

    Article

    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

    Insert

    hok/sok toxin-antitoxin cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC57KAN-40XALFA

    Plasmid

    #233118

    Purpose

    40X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57KAN-20XALFA

    Plasmid

    #233112

    Purpose

    20X ALFA tag multimer

    Depositor

    Steve Stowers

    Insert

    ALFA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57KAN-20XHA

    Plasmid

    #233114

    Purpose

    20X HA tag multimer

    Depositor

    Steve Stowers

    Insert

    HA tag
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19/mChe-Cs

    Plasmid

    #183171

    Purpose

    Construct for transient expression of cytoskeleton-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Cs
    

    Use

    Tags

    363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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