We narrowed to 4,318 results for: Pet2

1. pET21b - S. thermophilus DltB

   Plasmid

   #132607

   Purpose

   expresses S. thermophilus DltB in E. coli

   Depositor

   Wenqing Xu

   Article

   Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

   Insert

   Dltb
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAR69_STRI_m(KS_MAT)_H8_pET22b

   Plasmid

   #122849

   Purpose

   Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   m(KS_MAT) (Fasn Mouse)
   

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SpyTag002 DA-MBP

   Plasmid

   #102832

   Purpose

   Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002 DA-MBP
   

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Reactive aspartic acid has been mutated to an ala…

   Promoter

   T7

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a His6Flag2AUF1(p42)

   Plasmid

   #135941

   Purpose

   Expresses AUF1p42 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-PTBP1-RRM12CCtoSS

   Plasmid

   #89155

   Purpose

   Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
   

   Tags

   hexa His

   Expression

   Bacterial

   Mutation

   mutations: Cys 250 to Ser, Cys 251 to Ser

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 mSmo-Cterm

    Plasmid

    #58703

    Purpose

    used for anti-mouse Smoothened antibody production (for affinity purification)

    Depositor

    Matthew Scott

    Article

    Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

    Insert

    Smo (Smo Mouse)
    

    Tags

    6X-HIS

    Expression

    Bacterial

    Mutation

    contains C terminus (aa 543-793)

    Available Since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET29b-Ava3858-3857

    Plasmid

    #45744

    Depositor

    Christopher T Walsh, Emily Balskus

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3857
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET20b-delta133HtrA2 S400D

    Plasmid

    #16156

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S400D

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Tags

    Myc

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET20b-delta133HtrA2 G399S

    Plasmid

    #16158

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G399S

    Promoter

    T7

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pET29NK-LGK/mGFPmut3

    Plasmid

    #81168

    Purpose

    GFP fusion to levoglucosan kinase

    Depositor

    Timothy Whitehead

    Article

    Wrenbeck et al ACS Synth Biol. 2019 Mar 15;8(3):474-481

    Insert

    Levoglucosan kinase wild-type
    

    Tags

    His6x and mGFPmut3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b-delta133HtrA2 S142A

    Plasmid

    #16153

    Depositor

    L. Miguel Martins

    Article

    Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

    Insert

    HtrA2 (HTRA2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S142A

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only