We narrowed to 2,475 results for: T2A

1. pAAV CMV-hTau-T2A-EGFP-WPRE3-BGHpA

   Plasmid

   #240284

   Purpose

   The plasmid will package wild-type human Tau (hMAPT) as a T2A fusion with EGFP from a CMV promoter

   Depositor

   Bradley Hyman, Andrew Steele

   Insert

   hMAPT (MAPT Human)
   

   Use

   AAV

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E mCherry-T2A-hsHRAS-G12V-pA (JDW 1188)

   Plasmid

   #242571

   Purpose

   Gateway 3' entry clone containing mCherry followed by a T2A cleavage peptide and then human HRAS G12V.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   HRAS-G12V (HRAS Human)
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist Amp_d5-HT2AR(isoformD)-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221838

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoform D coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT2AR (isoform D) homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-HDR-donor

   Plasmid

   #221839

   Purpose

   Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   7xGFP11-HA donor with 5-HT2AR (isoforms BFH) homology arms
   

   Use

   CRISPR

   Expression

   Insect and Mammalian

   Available Since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF3

   Plasmid

   #241182

   Purpose

   Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

   Depositor

   Junsu Kang

   Insert

   NTRv2; mNeonGreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF1

   Plasmid

   #241180

   Purpose

   Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

   Depositor

   Junsu Kang

   Insert

   NTRv2; mNeonGreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. linker-T2A-NTRv2-linker-P2A-mNeonGreen ORF2

   Plasmid

   #241181

   Purpose

   Middle entry vector for mini-Golden subcloning platform; NTRv2; mNeonGreen

   Depositor

   Junsu Kang

   Insert

   NTRv2; mNeonGreen
   

   Use

   Mini-golden

   Mutation

   TCGC linker

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

   Plasmid

   #240229

   Purpose

   Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Norbert Perrimon, Justin Bosch

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

   Plasmid

   #238185

   Purpose

   Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   KLF4 (KLF4 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   mTagBFP2

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

    Plasmid

    #232936

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Podxl homology arms
    

    Use

    Mouse Targeting

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

    Plasmid

    #231907

    Purpose

    For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP

    Plasmid

    #227009

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

    Plasmid

    #227010

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

    Plasmid

    #227011

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

    Plasmid

    #227012

    Purpose

    Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, SV40 NLS

    Promoter

    UbC

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225588

    Purpose

    Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2(S54L+T127A)-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry

    Plasmid

    #225584

    Purpose

    Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2 (S54L+T127A)-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T21R-F8.2 (S54L+T127A)

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Promoter

    U6 and tp1

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid

    #221447

    Purpose

    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    SPR degron: hGEM (1-110) fragment that is fused t…

    Promoter

    CMV

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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