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Addgene

We narrowed to 2,007 results for: endog

Showing: 1181 - 1200 of 2007 results
  1. MLM3739

    Plasmid
    #49959
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Insert
    3x Flag Tet1CDmut HB-4 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. MLM3747

    Plasmid
    #49965
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Insert
    3x Flag Tet1CDmut HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  3. pJFA345D4

    Plasmid
    #49238
    Purpose
    TET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cells
    Depositor
    Insert
    TET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
    Use
    TALEN; Tale-tet1 expression vector
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
  4. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Expression
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. pJET-49-DExogron-mNeonGreen-R11a

    Plasmid
    #179903
    Purpose
    Donor with homologous arms for Rab11a to knock in DExogron (=minilAA7-DExCon) mNeonGreen module
    Depositor
    Insert
    RAB11A, member RAS oncogene family (RAB11A Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    minilAA7-mNeonGreen
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. LADL Bridge + Promoter Only Target

    Plasmid
    #127669
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter as well as 2 gRNAs targeting the Zfp462 promoter expressed from their individual hU6 promoters
    Insert
    CRY2-HA-2A-mCherry and gRNAs 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. qTAG-N-Solo-mStayGold-LMNB1

    Plasmid
    #227328
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)
    Depositor
    Insert
    LMNB1 Homology Arms flanking a mStayGold Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. qTAG-KO-Puro-TP53

    Plasmid
    #227319
    Purpose
    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)
    Depositor
    Insert
    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid
    #227278
    Purpose
    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Insert
    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. qTAG-C-Solo-mStayGold-ARL13B

    Plasmid
    #227277
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Insert
    ARL13B Homology Arms flanking a mStayGold Tag (ARL13B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. qTAG-C-mStayGold-EFS-Puro-CEP192

    Plasmid
    #227290
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Insert
    CEP192 Homology Arms flanking a mStayGold-EFS-Puro Cassette (CEP192 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. qTAG-N-Blast-moxGFP-LMNB1

    Plasmid
    #207772
    Purpose
    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Insert
    LMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. qTAG-N-Blast-miniTurbo-LMNB1

    Plasmid
    #207774
    Purpose
    Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Insert
    LMNB1 Homology Arms flanking a Blast-miniTurbo Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. qTAG-N-Solo-mScarlet-ACTB

    Plasmid
    #207750
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Insert
    ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1181 - 1200 of 2007 results