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pYPQ133B2.0

Plasmid

#99892

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.MTRIA-OT

Plasmid

#184594

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Promoter

Human synapsin promoter

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hAdipoq-GFP

Plasmid

#176215

Purpose

Expresses GFP under the control of distal enhancer and the promoter of human adiponectin gene

Depositor

Yu-Hua Tseng

Article

Shamsi et al Nat Commun. 2020 Mar 17;11(1):1421. doi:

Insert

GFP

Use

AAV

Expression

Mammalian

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Article

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pVDM1001

Plasmid

#134660

Purpose

CRISPR delivery and repair template delivery vector

Depositor

Willem van Schaik

Article

de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

Insert

CRISPR guide RNA array

Expression

Bacterial

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-AR

Plasmid

#111215

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

AR (Ar Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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UAS-3xHalo7::CAAX

Plasmid

#87646

Purpose

For creating drosophila transgenics expressing UAS-3xHalo7::CAAX

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

3xHalo7::CAAX

Expression

Insect

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pXCas9H840A

Plasmid

#60900

Purpose

Dual Expression Vector for Cas9 H840A nickase and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSPble

Plasmid

#63561

Purpose

Bleomycin drug selection marker

Depositor

Greg Matlashewski

Article

Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

Insert

Bleomycin drug selection marker

Use

Leishmania expression

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pDN80

Plasmid

#61349

Purpose

LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Type

Empty backbone

Tags

AsLOV2 wt and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pICH86966

Plasmid

#48075

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Nov. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pXL-BACII-LoxP-3xPDsRed-LoxP

Plasmid

#26852

Depositor

Christopher Potter

Insert

LoxP-3xPDsRed-LoxP

Expression

Insect

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-HyPer3

Plasmid

#119183

Purpose

AAV expression vector with CMV promoter driving expression of the hydrogen peroxide-sensitive fluorescent biosensor HyPer3.

Depositor

Thomas Michel

Article

Steinhorn et al Nat Commun. 2018 Oct 2;9(1):4044. doi: 1

Insert

HyPer3

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13

Plasmid

#110500

Purpose

Fluorescent Rab13

Depositor

Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab13 (RAB13 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-BRLF1

Plasmid

#72635

Purpose

Mammalian expression vector for Epstein-Barr virus Rta immediate-early replication and transcription activator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BRLF1

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pCDH-EF1-DIO-copGFP

Plasmid

#72253

Purpose

Express copGFP under EF-1 promoter when Cre is expressed by another vector

Depositor

Kazuhiro Oka

Insert

copGFP

Use

Cre/Lox and Lentiviral

Promoter

elongation factor-1

Available Since

Jan. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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ML1Nmut_in_pEGFP-C1

Plasmid

#67796

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pBluescript II SK--POLQ

Plasmid

#64873

Purpose

cloning vector for human POLQ

Depositor

Richard Wood

Article

Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

Insert

POLQ (POLQ Human)

Expression

Bacterial

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only

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