We narrowed to 6,128 results for: nls
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Intersectin I SH3 C domain (human)
Plasmid#47415DepositorInsertIntersectin SH3 domain C (ITSN1 Human)
UseTagsGSTExpressionBacterialMutationonly SH3 domain CPromotertacAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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CRY-tTA (B702)
Plasmid#92032PurposeExpresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activatorDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagstTA2 (TetR fused to VP64)ExpressionMammalianMutationNLS on CRY2 is mutatedPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN1735
Plasmid#127204PurposeDual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLSDepositorInsertpGal1-SynTF-mScarlet-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mouse Bmf
Plasmid#17244DepositorInsertBmf (Bmf Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
Intersectin I SH3 D domain (human)
Plasmid#47416DepositorInsertIntersectin SH3 domain D (ITSN1 Human)
UseTagsGSTExpressionBacterialMutationonly SH3 domain DPromotertacAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223 PAK5 T538N
Plasmid#60537PurposeDonor vector for human Pak5 T538N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationT538NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S573N
Plasmid#60538PurposeDonor vector for human Pak5 S573N mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS573NPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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iPEmax-WT
Plasmid#239385PurposeFor inverse prime editor using Cas9-R221K-N394K in HEK294T cellsDepositorInsertCas9-R221K-N393K, M-MLV-RT
UseCRISPRTagsBPNLS and SV40 NLS, c-myc NLSExpressionMammalianMutationR221K, N394K in Cas9PromoterCMVAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
iPEmax-D10A
Plasmid#239386PurposeFor inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cellsDepositorInsertnCas9-D10A-R221K-N394K, M-MLV-RT
UseCRISPRTagsBPNLS and SV40 NLS, c-myc NLSExpressionMammalianMutationD10A, R221K, N394K in Cas9PromoterCMVAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS1742
Plasmid#239838PurposeOrufIscB-KRK mammalian expressionDepositorInsertOrufIscB-KRK
UseTagsNucleoplasmin NLS-3xHA and SV40 NLSExpressionMammalianMutationInsertion of REC domain from NbaCas9-1 + E137K/E4…PromoterCMVAvailable sinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 12S->G
Plasmid#226597Purpose12 serines to glycinesDepositorInsertFUS LC 12S->A (FUS Human)
UseTags6HisExpressionBacterialMutation12 serines to alaninePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 12S->A
Plasmid#226598Purpose12 serines to alaninesDepositorInsertFUS LC 12S->A (FUS Human)
UseTags6HisExpressionBacterialMutation12 serines to alaninePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC StoG
Plasmid#226607PurposeAll serines to glycinesDepositorInsertFUS LC TtoS (FUS Human)
UseTags6HisExpressionBacterialMutationAll threonine to serinePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]
Plasmid#226766PurposeRab35(S22N)DepositorInsertrab35-mutant S22N (RAB35 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]
Plasmid#226764Purposerab35 (WT)DepositorInsertrab35 (RAB35 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]
Plasmid#226765PurposeRab35(Q67L)DepositorInsertrab35-mutant Q67L (RAB35 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB9v2
Plasmid#210029PurposeContains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puroDepositorInsertsCas9
rtta-T2A-puro
UseCRISPRTagsSV40 NLS and nucleoplasmin NLSExpressionMammalianMutationPromoterEF-1α and TRE3GAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB9
Plasmid#210036PurposeContains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFPDepositorInsertsCas9
puro-T2A-eGFP
UseCRISPRTagsSV40 NLS and nucleoplasmin NLSExpressionMammalianMutationPromoterEF-1α and TRE3GAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-CGBE1
Plasmid#198557PurposeExpresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHA850 - osm-10p::FynY531F
Plasmid#139207PurposeExpress activated Fyn kinase in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA852 - mec-4p::FynY531F
Plasmid#139209PurposeExpresses activated Fyn kinase in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_withR
Plasmid#139128Purposeexpress FUS LC with the same number and spacing of arginines as hnRNPA2 LCDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationadd argininesPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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hnRNPA2_LC_nocharge_wR
Plasmid#139119Purposeexpress hnRNPA2 LC with no charged residues with R added backDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationremove all K, most DPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_RtoK
Plasmid#139120Purposeexpress hnRNPA2 LC where all R are KDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall R to KPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_NtoS
Plasmid#139121Purposeexpress hnRNPA2 with all N changed to SDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall N to SPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_5E
Plasmid#139122Purposeexpress hnRNPA2 with 5 serine changed to glutamateDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutation5 serine to glutamatePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_12E
Plasmid#139123Purposeexpress hnRNPA2 with all serine change to glutamateDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutation12 S to EPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_YtoS
Plasmid#139124Purposeexpress hnRNPF PLD with all Y changed to SDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall Y to SPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_StoA
Plasmid#139125Purposeexpress hnRNPF PLD with all S changed to ADepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall S to APromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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BPK4387 - human expression plasmid for SpCas9 Cluster 5
Plasmid#101191PurposeHuman expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster5(K918A/V922A/R925A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationK918A/V922A/R925APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3645 - human expression plasmid for SpCas9 Cluster 3
Plasmid#101187PurposeHuman expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster3(T657A/G658A/W659A/R661A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationT657A/G658A/W659A/R661APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3770 - human expression plasmid for SpCas9 Cluster 4
Plasmid#101189PurposeHuman expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster4(F491A/M495A/T496A/N497A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationF491A/M495A/T496A/N497APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P50L
Plasmid#60533PurposeDonor vector for human Pak5 P50L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP50LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 P76T
Plasmid#60534PurposeDonor vector for human Pak5 P76T mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationP76TPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S312C
Plasmid#60535PurposeDonor vector for human Pak5 S312C mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationS312CPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V463L
Plasmid#60536PurposeDonor vector for human Pak5 V463L mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV463LPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 V593I
Plasmid#60539PurposeDonor vector for human Pak5 V593I mutantDepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationV593IPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only
