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pSP569
Plasmid#139499PurposeRepair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiaeDepositorInsertpSTL1 24xPP7sl STL1 100 - 600
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-rab10
Plasmid#109593PurposeMuscle specific zebrafish expression of mKate2 fused to rab10DepositorInsertrab10 (rab10 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-Tag/C448Y with 5'UTR
Plasmid#89318Purposeexpression of C448Y spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87C448Y
Plasmid#89329PurposeInducible expression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-460 bp deleted, C448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP C448Y with 5'UTR
Plasmid#89325PurposeInducible expression of C448Y spastin M1 and M87 in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutationC448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1C448Y
Plasmid#89326PurposeInducible expression of M1C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterTet-responsive P tightAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/S46X with 5'UTR
Plasmid#87729PurposeExpresses S46X spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationS46XPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/S44L with 5'UTR
Plasmid#87727PurposeExpresses S44L spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationS44LPromoterpCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/E112K with 5'UTR
Plasmid#87723PurposeExpresses E112K spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationE112KPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag/L195V with 5'UTR
Plasmid#87725PurposeExpresses L195V spastin M1 and M87 in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsExpressionMammalianMutationL195VPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag 3A/myc-M1E112K
Plasmid#87715PurposeExpresses myc-M1E112K spastin isoform in mammalian cellsDepositorInsertSPAST (SPAST Human)
UseTagsMycExpressionMammalianMutationM1 isoform; E112KPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVH004
Plasmid#80401PurposeContains pBAD-CusR and pCusR-antiscaffold with no fluorescent protein. Used as a control for autofluorescence.DepositorUseSynthetic BiologyTagsC-terminal LZx domainExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin
Plasmid#134860PurposeExpresses EGFP-tagged recycling endosome markerDepositorInsertRAB11B (RAB11B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.Halo-LDLR
Plasmid#162717PurposeMammalian expression of human LDLRDepositorInsertLDLR (LDLR Human)
UseTagsHaloExpressionMammalianMutationnonePromoterChicken Beta actinAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-Cux1
Plasmid#74341PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Cux1. Not for gene expression.DepositorInsertcut-like homeobox 1 (Cux1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA-FLAG-Bbs4
Plasmid#128868PurposeFor mammalian expression of mouse Bbs4DepositorInsertBbs4 (Bbs4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only
