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pGENE B::AtHAP2-V5

Plasmid

#132959

Purpose

Express AtHAP2 full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

AtHAP2::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pOpen-T3gene1

Plasmid

#165576

Purpose

DNA-dependent RNA polymerase derived from T3 bacteriophage that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T3 RNA Polymerase

Use

Synthetic Biology

Available Since

Aug. 24, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(0)_T2AGeneSwitch

Plasmid

#125214

Purpose

Artificial phase 0 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_061_gene10_5'UTR_T7

Plasmid

#235852

Purpose

gene10 5'UTR T7

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

gene10 5'UTR T7

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3a_001_gene10_5'UTR_T7

Plasmid

#235854

Purpose

gene10 5'UTR T7 3a

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

gene10 5'UTR T7

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJET-LB-geneticin

Plasmid

#213468

Purpose

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Left border of the TSI locus 1-split fragment of geneticin cassette

Expression

Bacterial

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pST_IGKV_ANDDA_Unigene43915 (pBS0336)

Plasmid

#185181

Purpose

For the mammalian expression of the Chinese giant salamander protein IGKV_ANDDA_Unigene43915. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

IGKV_ANDDA_Unigene43915

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGene-TAP-GBP1

Plasmid

#138087

Purpose

Plasmid with TAP-GBP1 ORF

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Tags

Tandem affinity purification (TAP) tag

Expression

Mammalian

Promoter

Gal4-E1b

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-FLAG-Gene33

Plasmid

#21633

Depositor

John Kyriakis

Article

Makkinje et al J Biol Chem. 2000 Jun 9. 275(23):17838-4

Insert

Gene33 (Errfi1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pLITMUS*-PBBa_J23106-geneIII

Plasmid

#134352

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene III

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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G608G lamin A minigene

Plasmid

#20292

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

G606G:GGC>GGT Mutation causes aberrant spli…

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pRK5 BARK1 minigene

Plasmid

#14695

Depositor

Robert Lefkowitz

Article

Koch et al J Biol Chem. 1994 Feb 25. 269(8):6193-7.

Insert

BARK1 minigene (GRK2 Bovine)

Tags

3'UTR of human b-globin

Expression

Mammalian

Mutation

BARK1 amino acids 495-689

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pEB2-mRuby3 Addgene

Plasmid

#104005

Purpose

Plasmid encoding mRuby3 Addgene

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRuby3 Addgene

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP611 (MSKGEE LIKENMRMKVV…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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