We narrowed to 15,787 results for: gRNA

1. lentiCRISPR - CTRL sgRNA

   Plasmid

   #70662

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a non-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   non-targeting sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHiUGE GS-gRNA (no Cre)

   Plasmid

   #200370

   Purpose

   Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpaCas12f1-gRNA_MS13

   Plasmid

   #190877

   Purpose

   The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ERN1 gRNA (BRDN0001162231)

   Plasmid

   #76409

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ERN1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ERN1 (ERN1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LRPPRC gRNA (BRDN0001145987)

   Plasmid

   #77773

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human LRPPRC

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   LRPPRC (LRPPRC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-P3-evoCjCas9-TadCDd_gRNA

   Plasmid

   #202563

   Purpose

   P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-TadCDd
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LRPPRC gRNA (BRDN0001147349)

   Plasmid

   #77774

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human LRPPRC

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   LRPPRC (LRPPRC Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PINK1 gRNA (BRDN0001147554)

   Plasmid

   #78035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. PINK1 gRNA (BRDN0001145357)

   Plasmid

   #78036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. TBK1 gRNA (BRDN0001148607)

    Plasmid

    #76363

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR v2 RB1 sgRNA #1

    Plasmid

    #202516

    Purpose

    Expressing Cas9 and sgRNA targeting human RB1 gene

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    RB1 sgRNA (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD1-dU6:1gRNA

    Plasmid

    #49408

    Purpose

    expression of gRNA under control of the Drosophila U6:1 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-1:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AKT1 gRNA (BRDN0001149414)

    Plasmid

    #75502

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AKT1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    AKT1 (AKT1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DR274-eGFP sgRNA

    Plasmid

    #61051

    Purpose

    for generation of a eGFP specific sgRNA from a T7 promoter

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Insert

    EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRIM28 gRNA (BRDN0001145369)

    Plasmid

    #76141

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TRIM28

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. px459-Hira KO sgRNA

    Plasmid

    #186938

    Purpose

    Hira KO in mouse ES cells

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    Hira KO sgRNA (Hira Mouse)
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid

    #86612

    Purpose

    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. eGFP L202 gRNA

    Plasmid

    #119132

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L202 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. EZH2 E10.3 gRNA

    Plasmid

    #90685

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human EZH2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    EZH2 (Guide Designation E10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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