We narrowed to 16,600 results for: gnal

1. pLenti-Cas9-NS-A

   Plasmid

   #207823

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EF-1a; U6

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW57.1-ZNF746-C571A

   Plasmid

   #207826

   Purpose

   Dox-inducible expression of ZNF746-C571A

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746-C571A (ZNF746 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   C571A

   Promoter

   TRE

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p667-UBC-ZNF746-V5-miniTurbo

   Plasmid

   #207820

   Purpose

   C-terminal tagged ZNF746 for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   ZNF746-V5-miniTurbo (ZNF746 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Expression

   Mutation

   Promoter

   UBC

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mEos3.2trLATo

   Plasmid

   #203746

   Purpose

   Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLATo (Lat Mouse)
   

   Use

   Tags

   ER translocation signal - mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

   Plasmid

   #192452

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   3x Nuclear localization signal (NLS) and mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LIC-Z-Y-L

   Plasmid

   #153546

   Purpose

   this is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucine

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   with 6 Y in 3 ITAMs mutated to L

   Promoter

   CMV

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CFFΔC-EGFP

   Plasmid

   #86059

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GST-cystin-CTS

   Plasmid

   #86066

   Purpose

   A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Cystin (Cys1 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 28-35

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CFFΔC-Myc

    Plasmid

    #86060

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    contains CD8a luminal and transmembrane domains a…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDNA3 T7 Jip1(1-470)

    Plasmid

    #51724

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 471-660

    Promoter

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDNA3 T7 Jip1(471-660)

    Plasmid

    #51766

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 1-470

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcdna3 T7Jip1(1-126)

    Plasmid

    #51702

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 127-660

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA3 T7 Jip1(1-281)

    Plasmid

    #51700

    Purpose

    " JIP-1 may suppress signal transduction by the JNK pathway"

    Depositor

    Roger Davis

    Article

    Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

    Insert

    Jnk interacting protein 1 (Mapk8ip1 Mouse)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    deleted 282-660

    Promoter

    Available since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-C-STAT1-mGFP-P2A-Puro

    Plasmid

    #199345

    Purpose

    express murine STAT1 protein fused with mGFP

    Depositor

    Massimo Mallardo

    Article

    Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

    Insert

    signal transducer and activator of transcription 1 (Stat1 Mouse)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-N1-STAT3

    Plasmid

    #111934

    Purpose

    Expresses human STAT3 fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

    Insert

    Signal transducer and activator of transcription 3 (STAT3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIE1

    Plasmid

    #136355

    Purpose

    Selection by Inducible Effectors

    Depositor

    Andrew Goodman

    Article

    Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

    Inserts

    Type VI effector bfe1
    Type VI effector bte1
    BT_4676
    tetR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    ATG removed to add signal sequence before gene

    Promoter

    P1TDP-GHO23

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-NLS-CBRed-d2-hygro

    Plasmid

    #139196

    Purpose

    non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBRed
    

    Use

    Luciferase and Retroviral

    Tags

    d2 PEST domain and nuclear localization signal (N…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL204

    Plasmid

    #22282

    Purpose

    Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV Syn CaMPARI2-human-a-Tubulin (TubuTag)

    Plasmid

    #164184

    Purpose

    Freeze-frame imaging of dendritic calcium signals CaMPARI2 moiety with lower Ca+ affinity (Kd=199nM) (Moeyaert et al., 2018)

    Depositor

    Thomas Oertner

    Article

    Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

    Inserts

    CamPARI2
    Human α Tubulin
    

    Use

    AAV

    Tags

    6 His tag and NES

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge