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We narrowed to 1,333 results for: Ncl

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Showing: 1221 - 1240 of 1333 results

  1. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

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  5. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid
    #194191
    Purpose
    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    4 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194191-plasmid-map-sequence-id-381776

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  6. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)

    Plasmid
    #194192
    Purpose
    Includes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    3 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194192-plasmid-map-sequence-id-381933

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  14. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  15. Ezh2[SET]-dCas9

    Plasmid
    #100087
    Purpose
    Catalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    Ezh2[SET] (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    Ezh2[SET] includes aa 482-746
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100087-plasmid-map-sequence-id-197556

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  17. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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  18. MKC62_HBB(HBA1reg-HBA1-2A-YFP)

    Plasmid
    #232408
    Purpose
    AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.
    Depositor
    Kyle Cromer
    Article
    Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0
    Insert
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Expression
    Mammalian
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232408-plasmid-map-sequence-id-466143

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  19. pDmelOR

    Plasmid
    #126476
    Purpose
    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    DmelOrco (Orco Fly)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens; includes a rec…
    Promoter
    CMV
    Available Since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126476-plasmid-map-sequence-id-246523

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Showing: 1221 - 1240 of 1333 results