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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,025 results for: PPL

Showing: 1221 - 1240 of 3025 results

  1. pKEK3238

    Plasmid
    #234092
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    sfCherry2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E117Q/T127I/G219A
    Promoter
    pJ23100-BsaI
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    234092-plasmid-map-sequence-id-467988

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  2. pKEK3228

    Plasmid
    #234090
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    aeBlue
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234090-plasmid-map-sequence-id-467993

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  3. pKEK3227

    Plasmid
    #234089
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    April 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    234089-plasmid-map-sequence-id-467986

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  5. CMV-O-GECO1

    Plasmid
    #46025
    Purpose
    Orange intensiometric genetically encoded Ca2+-indicators for optical imaging
    Depositor
    Robert Campbell
    Article
    Wu et al ACS Chem Neurosci. 2013 Mar 19.
    Insert
    O-GECO1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R-GECO1 N45I/A73V/S142P/M146R/M223T/M339L/K378R/E…
    Promoter
    CMV
    Available since
    July 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    46025-plasmid-map-sequence-id-311519

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  12. pBECKP-km

    Plasmid
    #117235
    Purpose
    A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
    Depositor
    Quanjiang Ji
    Article
    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii
    Insert
    ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    117235-plasmid-map-sequence-id-227412

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  13. pET-Trx-mSA2

    Plasmid
    #52320
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Use
    Tags
    FLAG, His, S tag, TEV site, and Thioredoxin
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52320-plasmid-map-sequence-id-84861

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  14. pCT5-bac1.8

    Plasmid
    #119871
    Purpose
    Constitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli
    Depositor
    Claudia Schmidt-dannert
    Article
    Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p
    Inserts
    CymR'
    sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    SNP in 592 bp
    Promoter
    PserA promoter and Pveg promoter with CuO sequence
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119871-plasmid-map-sequence-id-232788

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  16. pJMP8823

    Plasmid
    #220901
    Purpose
    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220901-plasmid-map-sequence-id-437054

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  17. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid
    #186310
    Purpose
    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction
    Depositor
    George Church
    Article
    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01
    Inserts
    sg61-PDGFR
    eGFP
    Use
    Tags
    His, myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186310-plasmid-map-sequence-id-366939

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Showing: 1221 - 1240 of 3025 results