We narrowed to 5,610 results for: SPI

1. pME Actin-Vhh sfGFP (JDW 1215)

   Plasmid

   #224495

   Purpose

   Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-sfGFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pME-3xNLS-mTagBFP2-Cre (JDW 918)

   Plasmid

   #224517

   Purpose

   A Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusion

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xNLS-mTagBFP2-Cre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-mScarlet3-Giantin (JDW 1356)

   Plasmid

   #224502

   Purpose

   Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet3-Giantin
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pME-EGFP-P2A-iCre (JDW 921)

   Plasmid

   #224519

   Purpose

   A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP-P2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME EFS-rtTA/rtTA3 (JDW 1230)

   Plasmid

   #224525

   Purpose

   A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS-rtTA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAdTrack-CMV HA ALAS-1

   Plasmid

   #22746

   Depositor

   Bruce Spiegelman

   Article

   Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

   Insert

   ALAS-1 (Alas1 Mouse)
   

   Use

   Adenoviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2010

   Availability

   Academic Institutions and Nonprofits only

7. p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

   Plasmid

   #224536

   Purpose

   A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG- NLS-mScarlet-I3
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-mTurquoise-P2A-iCre (JDW 920)

   Plasmid

   #224518

   Purpose

   A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mTurquoise-P2A-iCre
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pME-lox-3xSTOP-lox (JDW 869)

   Plasmid

   #224521

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked SV40 polyA, bGH polyA, and SV40 pA stop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LoxP-3xStop-LoxP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV_FLAG-NFL(R438TAG)

    Plasmid

    #182662

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position R438 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    R438TAG

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mScarlet-I-P2A-iCre (JDW 917)

    Plasmid

    #224516

    Purpose

    A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p5E-TetO8x-CMVmin (JDW 1087)

    Plasmid

    #224475

    Purpose

    Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TetO(8x)-CMV promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO-1 shRev-Erbalpha

    Plasmid

    #22747

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pET24a-VHH-tev-std

    Plasmid

    #109423

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE

    Plasmid

    #203847

    Purpose

    Flp-dependent expression of Chronos fused to EGFP

    Depositor

    Ivo Spiegel

    Article

    Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

    Insert

    Chronos
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO-1: shALAS-1

    Plasmid

    #22750

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shALAS-1 (Alas1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pAdTrack: shRev-Erbalpha

    Plasmid

    #22749

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Adenoviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pCMV_FLAG-NFL(K211TAG)

    Plasmid

    #182660

    Purpose

    Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
    

    Use

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    K211TAG

    Promoter

    CMV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge