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GFP-C1-CAMKIIalpha

Plasmid

#21226

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIalpha (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0001.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pLD1 translation factors

Plasmid

#117760

Purpose

BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 1-13

Use

Escherichia coli

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Expression

Worm

Promoter

sdhb-1

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF3

Plasmid

#232643

Purpose

transient expresses IRF3 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

IRF3 (IRF3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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LentiV2-EF1α-nCas9/RT

Plasmid

#210188

Purpose

Expression of nCas9/RT-P2A-Puro

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

nCas9/RT-P2A-Puro

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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FusionRed-pBAD

Plasmid

#54677

Purpose

Localization: Protein Expression Vector , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Type

Empty backbone

Tags

6xHis and FusionRed

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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LentiV2-hU6-evopreQ1

Plasmid

#210189

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET YBX-1

Plasmid

#13035

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

Insert

YBX1 (YBX1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only
Pflp-18::NGL-1::spGFP11

Plasmid

#65828

Purpose

For trans-synaptic labeling

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

NLG-1 (nlg-1 Nematode)

Tags

spGFP11

Expression

Worm

Mutation

N142S

Promoter

Pflp-18

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pFlpStop-attB-UAS-2.1-tdTom

Plasmid

#88910

Purpose

attB FlpStop plasmid, for insertion into MiMIC site

Depositor

Tom Clandinin

Article

Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

Insert

FlpStop

Expression

Insect

Promoter

5XUAS

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pc DNA FRT TO- APP695

Plasmid

#114193

Purpose

Creation of a stable cell line expressing APP mutation (AD in vitro study)

Depositor

Aleksandra Radenovic

Insert

Amyloid-Precursor-Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695: 695AA (2085bp, lack…

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-CAMKIIbeta(T287D)

Plasmid

#21223

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CAMKIIbeta(T287D) Lab plasmid CF 0001.

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB

Plasmid

#199305

Purpose

pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

CjPglB with flag tag

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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LentiV2-mU6-evopreQ1

Plasmid

#210190

Purpose

pegRNA/ngRNA library cloning

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Type

Empty backbone

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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