We narrowed to 2,026 results for: cas9 expression vector

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pGMF1-D

Plasmid

#242205

Purpose

AtU6-26p::sgRNA scaffold in L1 vector backbone, for subcloning of 1st 20 bp target sequence. For use in dicots.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 AtU6-26p::sgRNA1 for subcloning of 20 bp target sequence

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU6-26p

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Gal80

Plasmid

#169030

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6:3

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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BII-C3H

Plasmid

#133386

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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sgTrack-mCherry

Plasmid

#114013

Purpose

Empty sgRNA expression vector with co-expression of mCherry reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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sgTrack-GFP

Plasmid

#114012

Purpose

Empty sgRNA expression vector with co-expression of TurboGFP reporter

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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sgTrack-Gateway

Plasmid

#114011

Purpose

3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNA

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_BpiI-cloning-site_SV40polyA

Plasmid

#124547

Purpose

Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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L1p6_barley_sgRNA_scaffold

Plasmid

#231033

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 6

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p5_barley_sgRNA_scaffold

Plasmid

#231032

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 5

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p4_barley_sgRNA_scaffold

Plasmid

#231031

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 4

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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L1p3_barley_sgRNA_scaffold

Plasmid

#231030

Purpose

Cloning vector to clone sgRNA in scaffold under TaU6 promoter for Cas9 mediated CRISPR for goldengate level1 position 3

Depositor

Wilma van Esse

Article

Winkelmolen et al Plant Physiol. 2025 Aug 4;198(4):kiaf309

Insert

Scaffold for Cas9 mediated CRISPR

Use

CRISPR

Expression

Bacterial

Promoter

TaU6

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pL-SFFV.Reporter.RFP657.PAC

Plasmid

#61395

Purpose

Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-SlugABE-NNG

Plasmid

#214356

Purpose

Expresses SlugABE-NNG

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG

Use

AAV

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-SAM

Plasmid

#78905

Purpose

Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Aug. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-U63-tgRNA-Rev

Plasmid

#112811

Purpose

gRNA cloning vector for expressing 2-6 gRNAs based on tgFE design in Drosophila

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pPtGE35

Plasmid

#107999

Purpose

Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Cas9

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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lenticrispr-wt-ltr-puro

Plasmid

#173428

Purpose

Expresses Cas9 and guide RNA, contains intact LTR

Depositor

Jakob Reiser

Article

Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

Insert

S. pyogenes sgRNA cassette

Use

Lentiviral

Expression

Mammalian

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pPPED

Plasmid

#162468

Purpose

Destination Expression vector for dicot plant prime editing

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Inserts

Cas9H840A
attR gateway destination cassette

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine based sequence o…

Promoter

2x35S

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MKC91_tEPOR(UbC-GFP-BGH)

Plasmid

#232406

Purpose

AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus.

Depositor

Kyle Cromer, Matthew Porteus

Article

Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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