We narrowed to 21,453 results for: eng
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Plasmid#62520Purposewild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Drosha full length (DROSHA Human)
TagsGFPExpressionMammalianAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-EFS-MCP-3XBFPnls
Plasmid#75384PurposeMCP-3XBFPnlsDepositorInsertMCP
UseLentiviralTags3XBFPExpressionMammalianPromoterEFSAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_PA-Cre
Plasmid#122960PurposePhotoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpcDNA3.1_PA-Cre
UseCre/LoxExpressionMammalianPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH229: FCK-QuasAr1-mO2
Plasmid#51629PurposeFluorescent reporter for membrane voltage with improved brightnessDepositorInsertQuasAr1-mOrange
UseLentiviralTagsmOrange2ExpressionMammalianPromoterCaMKIIaAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_sfCherry2_TagBFP
Plasmid#83031PurposeExpresses full length sfCherry2 and TagBFP fusion in mammalian cellsDepositorInsertsfCherry+full length_TagBFP
UseLentiviralExpressionMammalianMutation3 mutations in sfCherry full length: E118Q, T128I…PromoterSFFVAvailable sinceJan. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAMD1-4
Plasmid#61565PurposeStandardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable sinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…MutationF70A/F108YAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA Flag Lamp2b-HA
Plasmid#71292PurposeFlag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.DepositorInsertLamp2b (LAMP2 Human)
TagsFlag, HA, and Lamp2 signal peptideExpressionMammalianPromoterCMVAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-mNeonGreen
Plasmid#99134PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoterDepositorInsertmNeonGreen
UseAAVExpressionMammalianPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH229: FCK-QuasAr1-mO2
Plasmid#51629PurposeFluorescent reporter for membrane voltage with improved brightnessDepositorInsertQuasAr1-mOrange
UseLentiviralTagsmOrange2ExpressionMammalianPromoterCaMKIIaAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pArk313
Plasmid#200257PurposeArk313 rep/capDepositorInsertArk313 Rep/Cap
UseAAVAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCJ190
Plasmid#162667PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
TagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.2 TDP-43 NLS1 YFP
Plasmid#84912PurposeMammalian expresion of TDP-43 NLS1 YFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTDP-43 (TARDBP Human)
TagsYFPExpressionMammalianMutationK82A, R83A, K84APromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpyTag003-MBP
Plasmid#133450PurposeExpresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasmDepositorInsertSpyTag003-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE6a
Plasmid#207851PurposeMammalian expression of PE6a prime editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE6a
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationEc48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNBU2_erm-TetR-P1T_DP-GH023
Plasmid#90324PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette with both the P1T_DP aTC-inducible promoter (RBS GH023) and the consitutively-driven TetR by the P2-A21 promoterDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by