168,238 results

1. pR6K-GFP-CASTIF

   Plasmid

   #199653

   Purpose

   R6K plasmid encoding a type I-F CAST but no CRISPR array

   Depositor

   IIya Finkelstein

   Insert

   CAST I-F systems
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pClodANL-FGF2

   Plasmid

   #107871

   Purpose

   Bacterial expression of FGF2-NanoLuc

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Insert

   FGF2
   

   Use

   Luciferase

   Tags

   NanoLuc

   Expression

   Bacterial

   Mutation

   wt sequence

   Promoter

   pro D

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CMV:Flag-Antares

   Plasmid

   #208852

   Purpose

   Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   Antares
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX-EF1a_GFP_CdTag-HA

   Plasmid

   #221492

   Purpose

   GFP lentiviral overexpression of C-terminal dTag fusion

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Syn1-mCherry

   Plasmid

   #222967

   Purpose

   Make lentivirus to express mCherry in syn1 expressing cells

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   synapsin I

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-ZDHHC18

   Plasmid

   #231666

   Purpose

   Expresses human ZDHHC18 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC18 (ZDHHC18 Human)
   

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEL685

    Plasmid

    #205592

    Purpose

    NEPE for prime editing in rice

    Depositor

    Yong Zhang

    Article

    Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    ZmUbi

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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