165,817 results

1. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lys-eMagB-iRFP

   Plasmid

   #162249

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   p18-eMagB-iRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GAPDH-CPG-TV

   Plasmid

   #70148

   Purpose

   a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
   

   Use

   Mouse Targeting and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Puro-miniAAVR-FLAG

   Plasmid

   #166717

   Purpose

   Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   miniAAVR-FLAG
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Only expressing PKD1-3 of ectodomain

   Promoter

   CMV

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_iETV2_P2A_GFP_Puro

   Plasmid

   #168805

   Purpose

   PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ETV2 (ETV2 Human, Synthetic)
   

   Use

   Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. eMagB-iRFP-Mito

   Plasmid

   #162250

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagB-iRFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-N2-Frankenbody-HA

   Plasmid

   #175292

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-HA frankenbody fused with mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBC-Pfv-Sapphire_PURO

    Plasmid

    #203651

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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