264,421 results

1. pCMV CEPIA2mt

   Plasmid

   #58218

   Purpose

   Green fluorescent indicator for calcium imaging in the mitochondria

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   CEPIA2mt
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GCaMP2 N263Y E282V M339L

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mt4xi-GCaMP6f

   Plasmid

   #192075

   Purpose

   Fluorescent reporter for mitochondrial calcium

   Depositor

   Timothy Ryan

   Insert

   4 repeats of improved COX8 signal peptide plus GCaMP6f
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAMBIA_ASX

   Plasmid

   #98888

   Purpose

   Plant expression vector for multigene cloning

   Depositor

   Kristina Gruden

   Article

   Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S (enhanced)

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. nCas9-PPE

   Plasmid

   #140445

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEYFP-N3-EPAC1

   Plasmid

   #113110

   Purpose

   Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

   Depositor

   Xiaodong Cheng

   Article

   Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

   Insert

   RapGEF3 (RAPGEF3 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   PCMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. human EB1 in pEGFP N1 to express EB1-GFP (JB131)

   Plasmid

   #39299

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Insert

   EB1 (MAPRE1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSV-PKI-v2

   Plasmid

   #45066

   Purpose

   Expression of PKI (PKA inhibitor)

   Depositor

   Richard Maurer

   Insert

   cAMP-dependent protein kinase inhibitor alpha
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-jAspSnFR3

    Plasmid

    #203488

    Purpose

    jAspSnFR3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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