Addgene

We narrowed to 2,134 results for: mt

Showing: 1861 - 1890 of 2134 results

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ATP6 DdCBE T2

Plasmid

#221953

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE A1

Plasmid

#221954

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE A2

Plasmid

#221955

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE C1

Plasmid

#221956

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE C2

Plasmid

#221957

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE G1

Plasmid

#221958

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE T1

Plasmid

#221960

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 alphaDdCBE T2

Plasmid

#221961

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CO1 DdCBE C1

Plasmid

#221962

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-CO1 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 DdCBE A1

Plasmid

#221946

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATP6 DdCBE A2

Plasmid

#221947

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Tags

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD-MTS2 (12)

Plasmid

#101698

Purpose

MTS2 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from β subunit of Nicotiana plumbaginifolia F1 ATPase]-AATG

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD-SOD2MTS (12)

Plasmid

#101692

Purpose

SOD2MTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Superoxide dismutase [Mn] (YHR008C)]-AATG

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2-ATPAMTS (12)

Plasmid

#101696

Purpose

ATPAMTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae ATP synthase subunit alpha (YBL099W)]-AATG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2-GLRX2MTS (12)

Plasmid

#101697

Purpose

GLRX2MTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Glutaredoxin-2 (YDR513W)]-AATG

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-Pygopus

Plasmid

#31900

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

Pygopus (pygo Fly)

Use

Tags

6xHIS and V5

Expression

Insect

Mutation

Promoter

P-MT (Metallothionein)

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

Plasmid

#176285

Purpose

Cre-dependent AAV virus

Depositor

Shenqin Yao, The Allen Institute for Brain Science

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA66T-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis-TEV-Nsp15

Plasmid

#187656

Purpose

E. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

Depositor

Debra Hansen

Article

Jernigan et al Structure. 2022 Dec 30:S0969-2126(22)004

Insert

Nsp15 (N Severe acute respiratory syndrome coronavirus 2)

Use

Tags

TEV protease cleavable His6-tag

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP5004 pAAV-Syn-DIO-TVA-dTom-SAD-B19G

Plasmid

#176286

Purpose

Cre-dependent AAV virus

Depositor

Shenqin Yao, The Allen Institute for Brain Science

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP5005 pAAV-Syn-DIO-TVA66T-dTom-SAD-B19G

Plasmid

#176287

Purpose

Cre-dependent AAV virus

Depositor

Shenqin Yao, The Allen Institute for Brain Science

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA66T-dTomato-rabies Glycoprotein

Use

Tags

dTomato

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP5002 RabV-SADdeltaG-histone-EGFP

Plasmid

#176284

Purpose

Recombinant rabies virus expressing histone-EGFP

Depositor

Shenqin Yao, The Allen Institute for Brain Science

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

H2B (H2BC11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
M-PKD2 (OF2-3)

Plasmid

#21370

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

wild-type*

Promoter

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
ColorfulCell

Plasmid

#62449

Purpose

MXS_chaining vector with 6 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMV::GTS-Citrine-bGHpA
CMV::Lyn-Ceruleanx3-bGHpA
CMV::NLS-TagBFPx3-bGHpA
CMV::MTS-AzamiGreen-bGHpA
CMV::iRFP670-SKL-bGHpA
CMV::ETS-mCherry-KDEL-bGHpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-2xMreBN-mCherry-His

Plasmid

#133626

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

2xMreB-mCherry-His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Xenopus APC-myc

Plasmid

#16686

Depositor

Barry Gumbiner

Article

Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

Insert

APC (apc.L Frog)

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
Codon Optimized Dysferlin

Plasmid

#67878

Purpose

The human dysferlin cDNA was codon optimized in an attempt to increase Dysferlin production in human cells.

Depositor

Matthew Hirsch

Article

Hirsch et al Mol Ther. 2013 Dec;21(12):2205-16. doi:

Insert

codon optimized Dysferlin

Use

Unspecified

Tags

No

Expression

Mutation

Promoter

No Promoter

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Use

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-Axin2

Plasmid

#21279

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only