We narrowed to 17,708 results for: URE

1. MMW3770 - human expression plasmid for SpCas9 Cluster 4

   Plasmid

   #101189

   Purpose

   Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   F491A/M495A/T496A/N497A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFOSGE_COF3H04

   Plasmid

   #70971

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Taf6 (Taf6 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFOSGE_COF1G05

   Plasmid

   #70809

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   CkIIbeta (CkIIbeta Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFOSGE_COF1B07

   Plasmid

   #70819

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   CkIIalpha (CkIIalpha Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

6. N4177 strain

   Bacterial Strain

   #13333

   Depositor

   Martin Gellert

   Article

   Menzel et al Cell. 1983 Aug . 34(1):105-13.

   Bacterial Resistance

   Coumermycin

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1/Ndi-1/myc-His

    Plasmid

    #127503

    Purpose

    Expresses myc-tagged yeast Ndi-1 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    Ndi-1 (NDI1 Budding Yeast)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIVM_26

    Plasmid

    #204501

    Purpose

    Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    0

    Promoter

    AmpR

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-BRD4 BD1

    Plasmid

    #196544

    Purpose

    Expression of BRD4 Bromodomain (BD1) in E.coli

    Depositor

    Byung Il Lee

    Article

    Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

    Insert

    BRD4 BD1 (BRD4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiGuide-Crimson

    Plasmid

    #70683

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    S. pyogenes sgRNA cassette
    Crimson Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-a and hU6

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-HA-ERK2 GOF

    Plasmid

    #53200

    Purpose

    Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S/D321N

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. His-MBP human SPOP (aa 28-337)

    Plasmid

    #52294

    Purpose

    bacterial expression of human SPOP (aa 28-337) fused to His-MBP

    Depositor

    Brenda Schulman

    Article

    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

    Insert

    SPOP (SPOP Human)
    

    Use

    Tags

    His-MBP and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

16. UPB-esophagus (UPB5)

    Plasmid

    #180783

    Purpose

    Unique Projection Barcode (UPB5)-Esophagus for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial ) (CHRM3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1/NDUFS3/myc-His

    Plasmid

    #127487

    Purpose

    Expresses myc-tagged NDUFS3 in mammalian cells

    Depositor

    Dieter Wolf, Shashi Jain

    Article

    Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

    Insert

    NDUFS3 (NDUFS3 Human)
    

    Use

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. (564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

    Plasmid

    #163025

    Purpose

    Expression of CRISPRi with gRNA against mouse Pcsk9

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. WDV-Echistatin

    Plasmid

    #226985

    Purpose

    Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

    Depositor

    Wendy Gordon

    Article

    Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

    Inserts

    WDV (repA )
    Echistatin
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Seattle_IPDA_control_9_003

    Plasmid

    #167349

    Purpose

    ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gag_tat_env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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