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pGEX-4T-1-p19-T7-GagB500

Plasmid

#46314

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
GagB500 DNA-1
linker
GagB500 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-A1T

Plasmid

#62256

Purpose

expression of A1T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A1T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126025

Purpose

express RFP- and mCer-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Pr-delRBS-deGFP

Plasmid

#67744

Purpose

Pr promoter expressing a deGFP that is missing a ribosomal binding site

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

deGFP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A4NT

Plasmid

#62261

Purpose

expression of A4NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A4NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a - NDH

Plasmid

#89626

Purpose

Expresses recombinant 6HIS tagged T. cruzi NADP-dependent oxidoreductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD(P)-dependent oxidoreductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3T

Plasmid

#62260

Purpose

expression of A3T sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3T sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-A3NT

Plasmid

#62259

Purpose

expression of A3NT sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

A3NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y91F

Plasmid

#47093

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y91F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pEN_TmiR_G13-L

Plasmid

#25762

Purpose

Entry vector with TRE promoter driving mouse G alpha 13 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Tags

myc-tag and twhh promoter (D rerio)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_AAVS1D

Plasmid

#183340

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1D

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1C

Plasmid

#183339

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1C

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC4

Plasmid

#183336

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC4

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC3

Plasmid

#183335

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC3

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC2

Plasmid

#183334

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC2

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_NTC1

Plasmid

#183333

Purpose

All-in-One CRISPRko system containing a non-targeting control

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

NTC1

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-gLuc-DR_pCMV-RedFireFly.gb

Plasmid

#242435

Purpose

Gaussia Luciferse with a Direct Repeat sequence in the 3’UTR recognised by the CasRx under the control of the CMV promoter, and a RedFirefly Luciferase driven by a CMV promoter.

Depositor

Diego Di Bernardo

Article

De Carluccio et al Nat Commun. 2024 Apr 17;15(1):3311. doi:

Insert

Gaussia Luciferase & Red Firefly Luciferase

Use

CRISPR and Luciferase

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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