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Viral Prep#112677-AAV5PurposeReady-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only
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pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV1)
Viral Prep#112677-AAV1PurposeReady-to-use AAV1 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV8)
Viral Prep#112677-AAV8PurposeReady-to-use AAV8 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)
Viral Prep#112677-AAV9PurposeReady-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)
Viral Prep#112677-AAV2PurposeReady-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry (Cre-negative cells), EGFP (Cre-positive cells)Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRISPRoff-mScarletI
Plasmid#217783PurposeExpresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencingDepositorInsertCRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
UseTags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletIExpressionMammalianMutationPromoterCAGAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCut plasmid toolkit
Plasmid Kit#1000000118PurposeCas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.DepositorAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX459-CTNNA1(α-catenin CRISPR KO)
Plasmid#229708PurposeKnockout of a-catenin in mammalian cells using CRISPR-Cas9 technologyDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-SF14P
Plasmid#209448Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from SF14P promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-kasOP*
Plasmid#209447Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoterDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from kasOP* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-PermE
Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from ermE* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAX6 CKO 2/7 Donor plasmid
Plasmid#68467Purposedonor plasmid for targeting PAX6 locusDepositorInsertFRT flanked exon4 of PAX6 gene (PAX6 Human)
UseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFREE
Plasmid#92050PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionBacterialMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITI2
Plasmid#166095PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPH3
Plasmid#166098PurposeGal inducible expression of D10A-Cas9 nickaseDepositorInsertCas9-D10A
UseCRISPRTagsExpressionYeastMutationspCas9-D10APromoterGal-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Zeo
Plasmid#92053PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Amp
Plasmid#92051PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Cm
Plasmid#92052PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRTagsExpressionMutationPromoterpRhamBAD and ptetAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only