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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 1261 - 1280 of 11938 results

  2. GLuc-PM

    Plasmid
    #164783
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors
    Depositor
    Laszlo Hunyady
    Publication
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    Tags
    GLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164783-plasmid-map-sequence-id-326675

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  3. GB-Dcp1b

    Plasmid
    #153979
    Purpose
    Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b
    Depositor
    Gia Voeltz
    Publication
    Lee et al Science. 2020 Jan 31;367(6477). pii: 367
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRNA decapping enzyme 1b (DCP1B Human)
    Tags
    pAcGFP-C1
    Expression
    Mammalian
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    153979-plasmid-map-sequence-id-306033

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  5. pEGFP-Rab21-T31N (DN)

    Plasmid
    #83423
    Purpose
    Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Publication
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab21 (Rab21 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residue 31 from T to N (T31N: ACG -> AAC)
    Promoter
    CMV
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83423-plasmid-map-sequence-id-159112

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  6. pG4-CMV-luc

    Plasmid
    #46322
    Depositor
    Steven Brown
    Publication
    Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xGal4
    Use
    Luciferase
    Tags
    CMV promoter and luciferase
    Expression
    Mammalian
    Mutation
    five copies of a multimerized GAL4 (5×GAL4) site …
    Promoter
    CMV
    Available since
    July 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. 7MEQ

    Plasmid
    #176412
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    8 x His Tag and Honeybee melittin signal
    Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    176412-plasmid-map-sequence-id-345854

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  12. EGFP-Rab8a-TN

    Plasmid
    #86077
    Purpose
    A mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hRab8A (RAB8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Changed threonine 22 to aspargine
    Promoter
    CMV
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86077-plasmid-map-sequence-id-168966

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  17. pMA-SpCas9-g3

    Plasmid
    #80786
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 3/13/23 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80786-plasmid-map-sequence-id-162406

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  19. pCDH-EF1-DIO-copGFP

    Plasmid
    #72253
    Purpose
    Express copGFP under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    copGFP
    Use
    Cre/Lox and Lentiviral
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72253-plasmid-map-sequence-id-131724

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Showing: 1261 - 1280 of 11938 results