We narrowed to 1,975 results for: 974

1. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

   Plasmid

   #199355

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199365

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc hSeparase

   Plasmid

   #59820

   Purpose

   Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-mGold-Mito-N-7

   Plasmid

   #158007

   Purpose

   Visualization of mitochondria in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEBB COMMD5 Flag

   Plasmid

   #74897

   Purpose

   Express COMMD5 in mammalian cells

   Depositor

   Colin Duckett, Ezra Burstein

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD5 (COMMD5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYLL204

   Plasmid

   #53455

   Purpose

   Expresses active site tyrosine mutated HA-tagged mouse DNA topoisomerase IIbeta Y814F (full length cDNA, in pCS2+ backbone)

   Depositor

   Leroy Liu, Lisa Lyu

   Article

   Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

   Insert

   HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) Y814F (Top2b Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y814F

   Promoter

   CMV

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

   Plasmid

   #98973

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 2 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mKO-Mito-7

   Plasmid

   #57848

   Purpose

   Localization: Mitochondria, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGEB-VirD2-Cas9

    Plasmid

    #139803

    Purpose

    Expressing VirD-Cas9 fusion under ubiquitin promoter

    Depositor

    Magdy Mahfouz

    Article

    Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

    Insert

    VirD2-Cas9
    

    Use

    Tags

    VirD2-Cas9

    Expression

    Plant

    Mutation

    Promoter

    Ubiquitin

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-FRMD6

    Plasmid

    #32838

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    FRMD6 (FRMD6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pFETCh_FOXP1

    Plasmid

    #86261

    Purpose

    Donor vector for 3' FLAG tag of human FOXP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    FOXP1 homology arms (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GST-GAR

    Plasmid

    #34697

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-145 of human fibrillarin with K2E and …

    Promoter

    tac

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

14. psgRNA (GB0645)

    Plasmid

    #68210

    Purpose

    Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX458_FOXP1_1

    Plasmid

    #86299

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199368

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOstar-ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-rtTA-zeo

    Plasmid

    #183233

    Purpose

    Lentiviral vector expressing the rtTa gene

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    rtTA-T2A-Zeo
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    UBC

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIneo-V5-SBP-HseIF4AII_AC

    Plasmid

    #148439

    Purpose

    Mammalian Expression of HseIF4AII

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AII (EIF4A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX458_FOXP1_2

    Plasmid

    #86300

    Purpose

    Encodes gRNA for 3' target of human FOXP1

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against FOXP1 (FOXP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge