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KJ801: pMVP/Hygro-DEST

Plasmid

#121858

Purpose

pMVP destination vector, empty expression vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL302: pMVP/Puro-DEST

Plasmid

#121856

Purpose

pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL302: pMVP/Puro-DEST

Plasmid

#121856

Purpose

pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_4

Plasmid

#26713

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to dog Rabin8 alpha isoform.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pLVX-TetOne-puro-atpG

Plasmid

#248067

Purpose

Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpG (atpG E. coli)

Use

Lentiviral

Tags

FLAG

Mutation

codon-optimized for expression in human cells

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-puro-atpG

Plasmid

#248067

Purpose

Lentiviral expression of gamma subunit of ATPGobble. Includes ampR and puroR (puromycin). Use together with pLVX-TetOne-bsd-atpA (#248065) and pLVX-TetOne-bleo-atpD (#248066) for active ATPGobble.

Depositor

Denis Titov

Article

Ekvik et al bioRxiv [Preprint]. 2025 Aug 23:2025.08.

Insert

atpG (atpG E. coli)

Use

Lentiviral

Tags

FLAG

Mutation

codon-optimized for expression in human cells

Promoter

TRE3GS

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R138Q)-TurboID

Plasmid

#245246

Purpose

Proximity-labeling proteomics with SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

TurboID

Expression

Mammalian

Mutation

R138Q

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R138Q)-TurboID

Plasmid

#245246

Purpose

Proximity-labeling proteomics with SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

TurboID

Expression

Mammalian

Mutation

R138Q

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R138Q)

Plasmid

#245228

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R138Q

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R138Q)

Plasmid

#245228

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R138Q

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510K)

Plasmid

#245235

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510K)

Plasmid

#245235

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R4A,R5A)

Plasmid

#245221

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(R4A,R5A)

Plasmid

#245221

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

R4A,R5A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T468M)

Plasmid

#245231

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T468M

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(T468M)

Plasmid

#245231

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

T468M

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(WT)-TurboID

Plasmid

#245242

Purpose

Proximity-labeling proteomics with SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Mar 21:2025.02.

Insert

SHP2 full-length

Tags

TurboID

Expression

Mammalian

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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