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Showing: 1281 - 1300 of 1418 results

  1. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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  3. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid
    #169182
    Purpose
    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    His6-3xFlag
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169182-plasmid-map-sequence-id-333999

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  4. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII

    Plasmid
    #217897
    Purpose
    Insect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tag
    Depositor
    Brenda Schulman
    Article
    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1
    Inserts
    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    Use
    Tags
    2xSTII
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    217897-plasmid-map-sequence-id-432185

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  7. pSN840

    Plasmid
    #184832
    Purpose
    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells
    Depositor
    Kyoko Chiba
    Article
    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g
    Inserts
    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    Use
    Cre/Lox
    Tags
    His-FLAG and mScarlet-2xStrepII
    Expression
    Insect
    Mutation
    ∆exon27 form of human KIF5A
    Promoter
    p10 and polyhedrin
    Available since
    July 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    184832-plasmid-map-sequence-id-367218

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  8. pDONR207 SARS-CoV-2 NSP1_nostop

    Plasmid
    #149304
    Purpose
    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149304-plasmid-map-sequence-id-296393

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  9. pDONR207 SARS-CoV-2 E_nostop

    Plasmid
    #149321
    Purpose
    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149321-plasmid-map-sequence-id-296470

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  11. pDONR207 SARS-CoV-2 ORF3A_nostop

    Plasmid
    #149319
    Purpose
    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149319-plasmid-map-sequence-id-296468

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  12. pDONR207 SARS-CoV-2 M_nostop

    Plasmid
    #149322
    Purpose
    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149322-plasmid-map-sequence-id-296471

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  13. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid
    #118412
    Purpose
    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.
    Depositor
    Nicole Paulk
    Article
    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1
    Insert
    Firefly luciferase
    Use
    AAV, Luciferase, and Synthetic Biology
    Tags
    Expression
    Insect and Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118412-plasmid-map-sequence-id-231800

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  14. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

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  15. pDONR207 SARS-CoV-2 ORF7A_nostop

    Plasmid
    #149324
    Purpose
    Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149324-plasmid-map-sequence-id-296473

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  16. pBFC0694

    Plasmid
    #186693
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_lacZ_α_1 gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186693-plasmid-map-sequence-id-371383

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  17. pDONR207 SARS-CoV-2 E 27nt-del

    Plasmid
    #153954
    Purpose
    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153954-plasmid-map-sequence-id-299760

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  19. pDONR207 SARS-CoV-2 E 27nt-del_nostop

    Plasmid
    #153955
    Purpose
    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 23, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153955-plasmid-map-sequence-id-300773

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  20. pEW108

    Plasmid
    #232823
    Purpose
    Expresses yeast compass complex in insect cells
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    Set1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232823-plasmid-map-sequence-id-465728

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Showing: 1281 - 1300 of 1418 results