We narrowed to 1,469 results for: MiCy

1. pDONR207 SARS-CoV-2 NSP3 Cys857Ala

   Plasmid

   #154027

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3
   

   Use

   Gateway-compatible entry vector, with mutagenized…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

   Plasmid

   #154029

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3
   

   Use

   Gateway-compatible entry vector, with mutagenized…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   June 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAcBac1-Mb-Fluoro-Phe B5

   Plasmid

   #197576

   Purpose

   Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

   Inserts

   Mb Pyl Fluoro-Phe B5 synthetase
   Pyl-tRNA (4 copies)
   

   Tags

   Nuclear export sequence + FLAG

   Expression

   Mammalian

   Mutation

   N311G, C313A

   Promoter

   CMV and U6/H1

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOmnibac_ZZ_linker_ybbR_MAP7

   Plasmid

   #159718

   Purpose

   Expresses MAP7 in Sf9 cells with a ZZ affinity tag and a ybbR tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   MAP7 (Microtubule Associated Protein 7) (MAP7 Human)
   

   Tags

   ZZ affinity tag, ybbR tag

   Expression

   Insect

   Available Since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPSV39-dddAI-FLAG

   Plasmid

   #186558

   Purpose

   Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

   Plasmid

   #169166

   Purpose

   Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

   Insert

   3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
   

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

   Plasmid

   #72922

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA Binding Protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Promoter

   TRE tight

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEN_TT MEK5-DD-mRFP1

   Plasmid

   #47547

   Purpose

   Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

   Insert

   MEK5-DD (MAP2K5 Human)
   

   Use

   Entry vector

   Tags

   mRFP

   Mutation

   S311D, T315D

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pEN_TTmiRC2_p53DD

   Plasmid

   #136520

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   p53DD (Trp53 Mouse)
   

   Use

   Entry vector for gateway cloning

   Tags

   3x FLAG

   Mutation

   This construct was created by PCR from the pBabe …

   Available Since

   Oct. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TT 3xHA-MKK6-EE

    Plasmid

    #47544

    Purpose

    Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

    Insert

    3xHA-MKK6-EE (MAP2K6 Human)
    

    Use

    Entry vector

    Tags

    3XHA

    Mutation

    S207E, T211E

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pACE-MBP-FBXL17 (310–701)

    Plasmid

    #228369

    Purpose

    express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

    Depositor

    Ning Zheng

    Article

    Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

    Insert

    FBXL17 (FBXL17 Human)
    

    Tags

    MBP

    Expression

    Insect

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Strep-His-Tev-hCGNL1

    Plasmid

    #236520

    Purpose

    For expression of Paracingulin (human) in insect cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (CGNL1 Human)
    

    Tags

    2xStrep-10xHis-Tev

    Expression

    Insect

    Mutation

    WT

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFB1.HMBP.PrS.CBX8ΔChromo

    Plasmid

    #224708

    Purpose

    Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 chromodomain truncation (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFBOH-MHL.mEGFP-CBX8

    Plasmid

    #224710

    Purpose

    Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Tags

    mEGFP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFB1.HMBP.PrS.CBX8-KR21A

    Plasmid

    #224709

    Purpose

    Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 KR21A mt (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1.HMBP.PrS.CBX8

    Plasmid

    #224707

    Purpose

    Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

    Insert

    CBX8 (CBX8 Human)
    

    Tags

    HMBP

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB1.HMBP.PrS.EZH2-mt1

    Plasmid

    #220239

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    R27A, R31A, F165A

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

    Plasmid

    #216378

    Purpose

    Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

    Depositor

    William Weis

    Article

    Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    HA signal sequence-FLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MultiMate-HITI-2c ACTB

    Plasmid

    #206267

    Purpose

    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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