We narrowed to 1,594 results for: PTS

1. pMECP2-GFP

   Plasmid

   #181904

   Purpose

   Fluorescently tagged MECP2, GFP

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   MECP2 (Mecp2 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. miR5 Mm ATP13A2

   Plasmid

   #171822

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. miR3 Mm ATP13A2

   Plasmid

   #171771

   Purpose

   transfer plasmid for lentiviral vector production with miR for Mm ATP13A2

   Depositor

   Veerle Baekelandt

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   ATP13A2 (Atp13a2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-6HIS-TEV-mEGFP,IRES-NGFR

   Plasmid

   #164521

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 2198

   Plasmid

   #49347

   Purpose

   This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

   Depositor

   Stephen Harris, Melissa Rogers

   Article

   Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

   Insert

   Bmp2 promoter (shorter sequence) (BMP2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Bmp2 promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMECP2-RFP

   Plasmid

   #181905

   Purpose

   Fluorescently tagged MECP2, RFP

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   MECP2 (Mecp2 Mouse)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE3G-ZSGreen1-mHP1alpha

   Plasmid

   #181913

   Purpose

   Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   ZSGreen1

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

   Plasmid

   #187576

   Purpose

   Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti HsATP13A2 D508N

    Plasmid

    #171820

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D508N

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT, D962N

    Promoter

    CMV

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti HsATP13A2 WT

    Plasmid

    #171485

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 WT

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D508N , D962N

    Promoter

    CMV

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti HsATP13A2 D962N

    Plasmid

    #171821

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 D962N

    Depositor

    Veerle Baekelandt

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT, D508N

    Promoter

    CMV

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid

    #164522

    Purpose

    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Insert

    NSP1 Delta RC (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert of NSP1 gene's CDS from SARS-CoV-2 is…

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-mHP1beta

    Plasmid

    #181901

    Purpose

    Fluorescently tagged HP1beta

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1beta (Cbx1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

    Plasmid

    #187580

    Purpose

    Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

    Plasmid

    #187577

    Purpose

    Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    FBL (FBL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYeDP60-hATP13A2-E343A

    Plasmid

    #171378

    Purpose

    A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

    Depositor

    Peter Vangheluwe

    Article

    van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

    Insert

    human ATP13A2 variant 2 wild-type
    

    Use

    Tags

    BAD tag

    Expression

    Yeast

    Mutation

    E343A

    Promoter

    URA3

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

    Plasmid

    #187586

    Purpose

    Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPHR-mCherry-hNCL

    Plasmid

    #181886

    Purpose

    Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNCL (NCL Human, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINSPECT:IL2_SigP:NLuc

    Plasmid

    #197266

    Purpose

    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

    Insert

    Interleukin-2 homology arms
    

    Use

    CRISPR, Luciferase, Synthetic Biology, and Unspec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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