We narrowed to 2,339 results for: crispr system

1. pYPQ-NF-L1-dMb2Cas12a

   Plasmid

   #177683

   Purpose

   Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   FokI-dMb2Cas12a
   

   Use

   CRISPR; Gateway compatible foki-dmb2cas12a entry …

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLSHiFi-P2A-Puro

   Plasmid

   #136902

   Purpose

   Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFi
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   R691A

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDIV302

   Plasmid

   #204973

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   nat1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV299

   Plasmid

   #204967

   Purpose

   AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

   Inserts

   Mad7
   argB
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Aspergillus nidulans tef1 promoter and native pro…

   Available since

   Nov. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SL3-MS2-gRNA GG backbone (MOBE3-4)

   Plasmid

   #219949

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-SL3 (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3'-com-gRNA GG backbone (MOBE1-4)

   Plasmid

   #219947

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-com-3'end (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3'MS2-gRNA GG backbone (MOBE1-2)

   Plasmid

   #219948

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-3'end (S. pyogenes Cas9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGA-pCLV3

   Plasmid

   #210528

   Purpose

   Cloning A. thaliana CLV3 promoter as GreenGate module A

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   pCLV3 (CLV3 Mustard Weed)
   

   Use

   Synthetic Biology; Golden gate compatible cloning…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-NF-L2-dMb2Cas12a

   Plasmid

   #177684

   Purpose

   Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

   Depositor

   Yiping Qi

   Article

   Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

   Insert

   FokI-dMb2Cas12a
   

   Use

   CRISPR; Gateway compatible foki-dmb2cas12a entry …

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-FNLSHF1-P2A-Puro

    Plasmid

    #136901

    Purpose

    Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHF1
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R661A,Q695A,Q926A

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-U6insert

    Plasmid

    #104536

    Purpose

    A piggybac vector with a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI) promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB4281

    Plasmid

    #98700

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCAR_Cas9-blast_GFP

    Plasmid

    #162074

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-blasticidin S deaminase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCAR_Cas9-hygro_GFP

    Plasmid

    #162075

    Purpose

    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Inserts

    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in M. musculus

    Promoter

    EF1a and sv40

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZDonor rosa26 LSL-mMALAT1

    Plasmid

    #140691

    Purpose

    generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region

    Depositor

    Jianjun Zhao

    Insert

    mouse MALAT1 (Malat1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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