We narrowed to 5,839 results for: pCas

1. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMUT2-pCaf-mCherry

   Plasmid

   #192860

   Purpose

   pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
   

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG eGFP-Oct4

   Plasmid

   #203789

   Purpose

   Competitive assay of Oct4 and Oct6 binding to DNA motifs.

   Depositor

   Thorsten Wohland

   Article

   Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   July 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-eArch3.0-mRuby2-ST

   Plasmid

   #109135

   Purpose

   Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eArch3.0-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-S-B.1.1.529-FLAG

   Plasmid

   #180774

   Purpose

   B.1.1.529 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.1.529
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 23, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCAG1.1-Myc/TSSK1

   Plasmid

   #199624

   Purpose

   Expression vector of mouse TSSK1. Myc-tag at N-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tssk1 mRNA (Tssk1 Mouse)
   

   Tags

   Myc-tag

   Promoter

   CAG

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9–mKate2ps–T1gRNA

   Plasmid

   #62717

   Purpose

   t1 gRNA (ATGAGAATCAAGGCGGTCGA)

   Depositor

   Leonidas Bleris

   Article

   Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

   Insert

   Cas9–mKate2ps
   

   Expression

   Mammalian

   Available Since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGEN-SBP-DICER1

   Plasmid

   #50558

   Purpose

   purification of Dicer1 from mammalian cells

   Depositor

   Yukihide Tomari

   Article

   Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

   Insert

   Dicer1 (Dicer1 Mouse)
   

   Tags

   SBP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. eSpCas9-EF-Gsk3b(new)

   Plasmid

   #122341

   Purpose

   Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Gsk3b
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9(1.1)_No_FLAG_ATP1A1_G2

    Plasmid

    #86610

    Purpose

    Expresses the ATP1A1 G2 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 exon 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-3xHAtag-SP-PTPRO

    Plasmid

    #191696

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of PTPRO
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1_G3

    Plasmid

    #86611

    Purpose

    Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-nDsRedFL-miRNT

    Plasmid

    #177808

    Purpose

    A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-NT
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-CRY2-mCherry

    Plasmid

    #178525

    Purpose

    Expresses CRY2-mCherry in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaSpeR4-tubulin-QF#7m1

    Plasmid

    #46128

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG GST DEPDC5

    Plasmid

    #164983

    Purpose

    DEPDC5 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    DEPDC5 (DEPDC5 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-MCS-mRuby2

    Plasmid

    #164093

    Purpose

    expression of mRuby2-tagged proteins under CAG promoter

    Depositor

    Maik Hintze

    Insert

    mRuby2
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS::AtHAP2-YFP

    Plasmid

    #132960

    Purpose

    Express AtHAP2 full length protein in mammalian cells

    Depositor

    Benjamin Podbilewicz

    Article

    Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

    Insert

    At HAP2::YFP
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-GFP-G3BP2(2)

    Plasmid

    #136061

    Purpose

    G3BP2 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GACAACTACTCCATCACTCA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP2 (G3BP2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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