We narrowed to 6,006 results for: pCas

Showing: 1281 - 1300 of 6006 results

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pCAG-Venus-MDGA1

Plasmid

#82543

Purpose

Overexpression vector for Venus-MDGA1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA1 (MDGA1 Human)

Tags

Venus fluorescent protein

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP7-p65AD

Plasmid

#49441

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP7-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-GFP

Plasmid

#184777

Purpose

Expresses H2B-GFP in human pluripotent stem cells

Depositor

Ivana Barbaric

Article

Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10

Insert

H2BC11 (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-pCaMKII-tTAc

Plasmid

#172125

Purpose

Expression of InteinC-tTAC in CaMKII positive cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

pCaMKII, tTAc

Use

AAV

Promoter

CaMKII

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-GST FNIP2

Plasmid

#164982

Purpose

FNIP2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FNIP2 (FNIP2 Human)

Tags

GST

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCA528 AMSH 1-178

Plasmid

#160050

Purpose

Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

AMSH residues 1-178 (STAMBP Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-178

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCaST-elav-VP16AD

Plasmid

#15308

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

VP16AD-ZIP+

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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Cav2.2 pHfluorin pCAGGS

Plasmid

#206073

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial pHfluorin tag in domain II

Depositor

Annette Dolphin

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–dSpCas9

Plasmid

#157833

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFREE2-SpCas9-sgRNA

Plasmid

#179582

Purpose

Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

Cas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Ace2_D92N_E199V-mNeon-ST

Plasmid

#129261

Purpose

Express soma-localized Ace2_D92N_E199V-mNeon in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace2_D92N_E199V-mNeon-ST

Expression

Mammalian

Mutation

D92N, E199V

Promoter

CAG

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-nucGFP11-3xControlgRNA

Plasmid

#224568

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

GFP11-H2B

Use

CRISPR

Tags

Histone H2B (nuclear localization)

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-C1V1-TT-mRuby2-ST

Plasmid

#109124

Purpose

Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

C1V1-TT-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT52

Plasmid

#218725

Purpose

Expresses N-terminally EGFP-tagged IFT52 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

Insert

IFT52 (IFT52 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTrex-b-NLS-hSpCas9

Plasmid

#62543

Purpose

Expression of Cas9 in T. cruzi

Depositor

Rick Tarleton

Article

Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

Insert

NLS-hSpCas9

Use

CRISPR; Trypanosoma cruzi expression

Tags

3xFLAG and NLS

Promoter

Ribo-HX1

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-MDGA2

Plasmid

#82544

Purpose

Overexpression vector for Venus-MDGA2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

MDGA2 (Mdga2 Rat)

Tags

Venus fluorescent protein

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-NL-DHFR-SpCas9

Plasmid

#124522

Purpose

Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

Depositor

Amit Choudhary

Article

Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

Insert

NL-DHFR-SpCas9

Use

AAV

Tags

Destabilized domain of E.coli dihydrofolate reduc…

Expression

Mammalian

Promoter

Cbh

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#1

Plasmid

#107726

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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