163,743 results

1. R777-E140 Hs.NF1-nostop

   Plasmid

   #70424

   Purpose

   Gateway ORF clone of codon optimized human NF1 [NM_000267.3] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   NF1 (NF1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

   Plasmid

   #211469

   Purpose

   Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ATAD3A (ATAD3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

   Viral Prep

   #137134-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

4. pEGFP_sfCherry2(11)_HP1

   Plasmid

   #82607

   Purpose

   Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_HP1b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pAAV.TBG.PI.Null.bGH

   Plasmid

   #105536

   Purpose

   empty AAV vector with TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   None
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT3TS-spRYCas9-ABE-UMax

   Plasmid

   #222139

   Purpose

   Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   TadA-nSpRYCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-Rab7a-7

   Plasmid

   #55127

   Purpose

   Localization: Ras GTPase, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   N117S in NM_004637.5

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-neo-(HA)3-ubiquitin

   Plasmid

   #196991

   Purpose

   Expresses HA-tagged ubiquitin in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

   Insert

   ubiquitin (UBC Human)
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent mutation in ubiquitin codon 13

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Venus-CAAX

   Plasmid

   #87612

   Purpose

   Plasma membrane-targeted Venus (YFP).

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   Venus-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-LacR-TopBP1

    Plasmid

    #31317

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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