We narrowed to 10,313 results for: Rras

1. Stargazin-mCherry-FRB

   Plasmid

   #172443

   Purpose

   Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Cacng2 (Cacng2 Mouse)
   

   Use

   Tags

   mCherry-HA-FRB

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAF0034 Ef1a-Kp03-2a-EGFP

   Plasmid

   #193461

   Purpose

   Expression of Kp03 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAF0036 Ef1a-Si74-2a-EGFP

   Plasmid

   #193463

   Purpose

   Expression of Si74 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Si74
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAF0432 Ef1a-Dn29-2a-EGFP

   Plasmid

   #193459

   Purpose

   Expression of Dn29 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAF0035 Ef1a-Nm60-2a-EGFP

   Plasmid

   #193464

   Purpose

   Expression of Nm60 recombinase from Ef1a promoter

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Nm60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT7-EGFP-C1-HsEDC3

   Plasmid

   #25032

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   EDC3 (EDC3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCIneo-V5-SBP-HseIF4AI_AC

   Plasmid

   #148438

   Purpose

   Mammalian Expression of HseIF4AI

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HseIF4AI (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   3 silent mutations relative to NM_001416.2

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTol2-elavl3-WHaloCaMP1a-EGFP

   Plasmid

   #205311

   Purpose

   Expression of WHaloCaMP1a-EGFP in zebrafish from the elavl3 promoter (pan-neuronal expression)

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a-EGFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAF0084 Ef1a-Cp36-2a-EGFP

    Plasmid

    #193462

    Purpose

    Expression of Cp36 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLNHA-C1-Hs4E-BP1

    Plasmid

    #79438

    Purpose

    Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    Hs4E-BP1 (EIF4EBP1 Human)
    

    Use

    Tags

    lambdaN-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CaMKII-WHaloCaMP1a

    Plasmid

    #205310

    Purpose

    Production of AAV particles encoding WHaloCaMP1a from the CaMKII promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

    Plasmid

    #184951

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Patrick Hsu, Ami Bhatt, Michael Bassik, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT7-EGFP-C1-HsRCK

    Plasmid

    #25033

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    Rck (DDX6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-MS2-HA-HsRck_AC

    Plasmid

    #148417

    Purpose

    Mammalian Expression of HsRck

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HsRck (DDX6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hCD4-mOrange

    Plasmid

    #110192

    Purpose

    Encodes for human CD4 coding sequence tagged with the mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Iliopoulou et al Nat Struct Mol Biol. 2018 Sep;25(9):814-

    Insert

    hCD4 (CD4 Human)
    

    Use

    Tags

    mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 HisC EXO1b

    Plasmid

    #68268

    Purpose

    Mammalian expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pEYFP-N1-A2BR

    Plasmid

    #37202

    Depositor

    Robert Tarran

    Article

    Watson et al FASEB J. 2011 Sep;25(9):2996-3003. Epub

    Insert

    A2BR (ADORA2B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pL1M-pMtRC-MtLAP1-Adh-R1

    Plasmid

    #170790

    Purpose

    A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

    Depositor

    Jeremy Murray

    Article

    Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

    Insert

    MtLAP1 (LOC11412013 Medicago truncatula)
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Medtr4g059670(MtRC)

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAF0362 Ef1a-Cp36_attP-Puro-p2a-mCherry donor

    Plasmid

    #193469

    Purpose

    Cp36 Donor with promoter for mcherry and puro expression

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTracer_Cr-TRP1

    Plasmid

    #64881

    Purpose

    Expresses CrTRP1 in mammalian cells

    Depositor

    Sebastián Brauchi

    Article

    Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

    Insert

    Transient receptor potential 1
    

    Use

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Patrick Hsu, Ami Bhatt, Michael Bassik, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

    Plasmid

    #130708

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETM-41-HsPABPC1_J

    Plasmid

    #146644

    Purpose

    Bacterial Expression of HsPABPc1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    HsPABPc1 (PABPC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pT7-EGFP-HseIF4E

    Plasmid

    #79437

    Purpose

    Expresses EGFP-tagged HseIF4E in Mammalian cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

    Insert

    HseIF4E (EIF4E Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hCCR5-LSSmOrange

    Plasmid

    #110194

    Purpose

    Encodes for human CCR5 coding sequence tagged with the LSS-mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCCR5 (CCR5 Human)
    

    Use

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT7-EGFP-C1-HsNXF-1-5xmut_O

    Plasmid

    #147116

    Purpose

    Mammalian Expression of HsNxf-1-5xmut

    Depositor

    Elisa Izaurralde

    Article

    Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

    Insert

    HsNxf-1-5xmut (NXF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Actin binding domain of Ezrin

    Plasmid

    #62381

    Purpose

    Expresses the actin binding domain of ezrin. Amino acids 552-586.

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Actin binding domain of ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains aa's 552-586

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-V5-SBP-C1-Hs4EBP1_Z

    Plasmid

    #148150

    Purpose

    Mammalian Expression of Hs4EBP1

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    Hs4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCIneo-V5-SBP-MBP-HsNot4_AG

    Plasmid

    #148763

    Purpose

    Mammalian Expression of HsNot4

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-mGrn-myc

    Plasmid

    #178944

    Purpose

    Construct for expressing murine progranulin (Grn) in AAV

    Depositor

    Erik Roberson

    Article

    Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

    Insert

    Grn (Grn Mouse)
    

    Use

    AAV

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTol2-elavl3-WHaloCaMP1a

    Plasmid

    #205312

    Purpose

    Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT7-EGFP-C1-HsAGO2_F

    Plasmid

    #146315

    Purpose

    Mammalian Expression of HsAGO2

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsAGO2 (AGO2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation C840T and sequence differs in…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCIneo-lambdaN-HA-HsDCP2-E148Q_U

    Plasmid

    #147643

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT7-MS2-HA-C1-MBP-DmBam_13-36_AG

    Plasmid

    #148839

    Purpose

    Mammalian Expression of DmBam_13-36

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_13-36 (bam Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

    Plasmid

    #130709

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCIneo-V5-SBP-HseIF4AII_AC

    Plasmid

    #148439

    Purpose

    Mammalian Expression of HseIF4AII

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AII (EIF4A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pnEA-NpH-HsNot9_S

    Plasmid

    #147494

    Purpose

    Bacterial Expression of HsNot9

    Depositor

    Elisa Izaurralde

    Article

    Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

    Insert

    HsNot9 (CNOT9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLNHA-C1-HsTTP_1-313_AH

    Plasmid

    #148879

    Purpose

    Mammalian Expression of HsTTP_1-313

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsTTP_1-313 (ZFP36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCIneo-V5-SBP-MBP-HsNot4_64-575_AG

    Plasmid

    #148766

    Purpose

    Mammalian Expression of HsNot4_64-575

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4_64-575 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pT7-EGFP-C1-HsGIGYF1_AE

    Plasmid

    #148569

    Purpose

    Mammalian Expression of HsGIGYF1

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF1 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCIneo-lambdaN-HA-HsSmg6_I

    Plasmid

    #146551

    Purpose

    Mammalian Expression of HsSmg6

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6 (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations T594C, A699G and two non sil…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pT7-V5-SBP-C1-Hs4EBP3_Z

    Plasmid

    #148151

    Purpose

    Mammalian Expression of Hs4EBP3

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    Hs4EBP3 (EIF4EBP3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pT7-EGFP-C1-HsDCP2_1-395_V

    Plasmid

    #147808

    Purpose

    Mammalian Expression of HsDCP2_1-395

    Depositor

    Elisa Izaurralde

    Article

    Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

    Insert

    HsDCP2_1-395 (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

    Plasmid

    #146553

    Purpose

    Mammalian Expression of HsSmg6-del36-576-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsSmg6-del36-576-siRNAres (SMG6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pONSY-Lifeact:mCherry

    Plasmid

    #111876

    Purpose

    This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Lifeact fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pONSY-Venus

    Plasmid

    #111875

    Purpose

    This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCas9GG

    Plasmid

    #131009

    Purpose

    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Inserts

    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_R0040 TetR

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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