We narrowed to 15,977 results for: LEA

1. pCS2+-FLAG3-hsAgo2D-A

   Plasmid

   #221720

   Purpose

   Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2D-A (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Mutation

   D669A in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+-FLAG3-hsAgo2

   Plasmid

   #221719

   Purpose

   Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+-FLAG3-drAgo2Y-F

   Plasmid

   #221715

   Purpose

   Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2Y-F (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Mutation

   Y680F in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-FLAG3-drAgo2D-E

   Plasmid

   #221714

   Purpose

   Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drAgo2D-E (ago2 Zebrafish)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Mutation

   D651E in active site

   Promoter

   simian CMV IE94

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMBS-859

   Plasmid

   #218009

   Purpose

   mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   mCerulean
   

   Use

   Synthetic Biology

   Tags

   Factor Xa protease cleavage site and Strep-tag II

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HeFm1SpCas9

   Plasmid

   #92110

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. hFLT1

   Plasmid

   #83435

   Purpose

   human VEGFR1 with HA and FLAG tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Exp Cell Res. 2013 Oct 15;319(17):2578-8

   Insert

   human VEGFR1 (FLT1 Human)
   

   Tags

   Flag and Myc

   Expression

   Mammalian

   Mutation

   HA-tag was inserted 30 AA downstream of the FLT1 …

   Promoter

   CMV

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-Flag-MIB1-IRES-Puro

   Plasmid

   #140240

   Purpose

   Lentiviral vector expressing flag-tagged MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   Flag-MIB1 (MIB1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. rAAV-U6-MeCP2shRNA-CamKIIa-mCherry

    Plasmid

    #169704

    Purpose

    MeCP2 Knockdown

    Depositor

    Ana Oliveira

    Article

    Gulmez Karaca et al Neurobiol Learn Mem. 2018 Mar;149:84-97.

    Insert

    shRNA against MeCp2 (Mecp2 Mouse)
    

    Use

    AAV

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-EXO1a

    Plasmid

    #111629

    Purpose

    Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

    Insert

    Exonuclease 1a (EXO1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-GFAP-eGFP-shLacZ

    Plasmid

    #223222

    Purpose

    mir30 based shRNA strategy, control shRNA

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shLacZ
    

    Use

    AAV

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTXB1-EXO1b D173A

    Plasmid

    #68269

    Purpose

    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

    Depositor

    Stefano Ferrari

    Article

    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    catalytically-dead D173A. Please see depositor co…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pNUT N6His K206E/K534A hTFNG

    Plasmid

    #70133

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.

    Insert

    N6His K206e/K534A hTFNG (TF Human)
    

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    changed Lys206 to a glutamic acid and Lys534 to a…

    Promoter

    SV40

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-SpCas9-HF1 (without sgRNA)

    Plasmid

    #92102

    Purpose

    Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Drosha270-1374delNLS2

    Plasmid

    #62532

    Purpose

    N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    truncated human Drosha mutant (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-269 and nuclear localizatio…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

19. hFlt1 Y1333F

    Plasmid

    #86037

    Purpose

    human VEGFR1 with Y to F mutation at position 1333

    Depositor

    Christie Thomas

    Article

    Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

    Insert

    human Flt1 Y1333F (FLT1 Human)
    

    Tags

    FLAG, HA, and Myc

    Expression

    Mammalian

    Mutation

    Y1333F; HA tag is inserted downstream of signal …

    Promoter

    CMV

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. hFLT1 delta 754-763

    Plasmid

    #86035

    Purpose

    human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

    Insert

    human Flt1 delta754-763 aa (FLT1 Human)
    

    Tags

    FLAG, HA, and Myc

    Expression

    Mammalian

    Mutation

    delta 754-763

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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