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We narrowed to 1,598 results for: Tef

Showing: 1301 - 1320 of 1598 results

  1. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Use
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  4. pCAS9i

    Plasmid
    #141249
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141249-plasmid-map-sequence-id-308874

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  12. pHOHO2_Hsp82viiA

    Plasmid
    #61708
    Purpose
    Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.
    Depositor
    Dan Bolon
    Article
    Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:
    Insert
    Hsp82viiA (HSP82 Budding Yeast)
    Use
    Yeast integration vector targeting inserts to the…
    Tags
    6xHis
    Expression
    Yeast
    Mutation
    Heteromeric coiled coil inserted after amino acid…
    Promoter
    TEF1
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    61708-plasmid-map-sequence-id-104523

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  15. pcDNA3.1-MBII85-5'SS mut

    Plasmid
    #67631
    Purpose
    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.
    Depositor
    Stefan Stamm
    Article
    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00
    Insert
    MBII85 snoRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutation in 5'ss
    Promoter
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    67631-plasmid-map-sequence-id-116897

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  17. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  18. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

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  20. pMM0646

    Plasmid
    #129007
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129007-plasmid-map-sequence-id-259441

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Showing: 1301 - 1320 of 1598 results