We narrowed to 8,983 results for: ef1;

1. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

   Plasmid

   #159756

   Purpose

   PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

   Plasmid

   #105715

   Purpose

   In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

   Depositor

   Rylan Larsen

   Has Service

   AAV5

   Insert

   synaptophysin-GCaMP6s
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

   Plasmid

   #71216

   Purpose

   dCas9 fused to human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A catalytic domain
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-EF1-IRES-NEO mutant ITR

   Plasmid

   #67905

   Purpose

   PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-FLuc-WPRE-HGHpA

   Plasmid

   #87951

   Purpose

   Recombinant single-stranded AAV transfer vector expressing Firefly Luciferase under the EF1a promoter

   Depositor

   Mark Kay

   Article

   Paulk et al Mol Ther. 2017 Sep 25. pii: S1525-0016(1

   Insert

   Firefly Luciferase
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 61426-hU6-shmFmr1-EF1a-mCherry

   Plasmid

   #157859

   Purpose

   Expresses a shRNA targeting mouse Fmr1 gene

   Depositor

   Xinyu Zhao

   Article

   Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

   Insert

   Fmr1 (Fmr1 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1-FLP-PGK-Neo

   Plasmid

   #108544

   Purpose

   Lentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoter

   Depositor

   Javier Alcudia

   Inserts

   flp recombinase
   Neomycin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   EF1apha and PGK

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

   Plasmid

   #39455

   Depositor

   Hongjun Song

   Article

   Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

   Insert

   HA-hTet1CDmu (TET1 Human)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   aa1418–2136 with mutations H1672 to Y, 1674D to A

   Promoter

   EF1a

   Available since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-EF1a-FDIO-ArchT-GFP

    Plasmid

    #124640

    Purpose

    AAV-mediated FLPo-dependent expression of ArchT-GFP under EF1a promoter

    Depositor

    David Dupret

    Article

    Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

    Insert

    ArchT
    

    Use

    AAV; Flp/frt

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-Ef1a-RPS27A-UTR-PP7-WPRE

    Plasmid

    #198339

    Purpose

    PB plasmid expressing RPS27A with its 3'UTR tagged to PP7 stem loops

    Depositor

    Michael Ward

    Article

    De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

    Insert

    RPS27A-3'UTR-PP7 stem loops x24
    

    Use

    Piggybac

    Tags

    PP7 stem loops

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

    Plasmid

    #71215

    Purpose

    dCas9 fused to EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-EGFP
    

    Use

    Lentiviral

    Tags

    dSpCas9-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1a-mTagBFP-T2A-TetOn3G

    Plasmid

    #179888

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    mTagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pb459-mU6-sgRNA-EF1a-PuroR

    Plasmid

    #195507

    Purpose

    piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    Puromycin
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-FLEX-ArchT-GFP

    Plasmid

    #58851

    Purpose

    AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQLL-Tc1-8B_DR[EF1α-copGFP]

    Plasmid

    #218327

    Purpose

    Donor plasmid of Tc1-8B_DR Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

    Plasmid

    #122102

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-EF1α-IRES-EGFP-hMCIDAS

    Plasmid

    #149696

    Purpose

    Expresses untagged human MCIDAS in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Insert

    MCIDAS (MCIDAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1 alpha

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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