We narrowed to 6,369 results for: wus;

1. pCMV-PEmax-P2A-hP53DD

   Plasmid

   #214084

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-hP53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PEmax-P2A-hP53DD (TP53 Human, SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p23-mNeongreen-DDX21 DAT-mRuby3

   Plasmid

   #179226

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen and mRuby3

   Expression

   Mammalian

   Mutation

   G234D, K235A

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTarget-RFP-GFP operon

   Plasmid

   #192279

   Purpose

   RFP GFP fusion protein expressed under a constitutive promoter

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   RFP GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacIq

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

   Plasmid

   #117856

   Purpose

   This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

   Depositor

   C. Ron Yu

   Article

   Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

   Insert

   Kir2.1 (Kcnj2 Mouse)
   

   Use

   Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-mVenus-PA-Rac1-C450A

   Plasmid

   #22021

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV domain contains the light-insensitive C450A m…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

   Plasmid

   #22034

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450M.Rac1 s…

   Promoter

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. p23-mNeongreen-DDX21 HGV-mRuby3

   Plasmid

   #179227

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Use

   Tags

   mNeongreen and mRuby3

   Expression

   Mammalian

   Mutation

   D338H, E339G

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

   Plasmid

   #22023

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450A.Rac1 s…

   Promoter

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pSIN-CD4

   Plasmid

   #175942

   Purpose

   knockout mouse CD4

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   CD4 gRNA (Cd4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pE-cadherin-GFP-N-WASP deltaVCA

    Plasmid

    #101283

    Purpose

    Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    delta VCA domain

    Promoter

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Use

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Promoter

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-mNeongreen-DDX21 HGV

    Plasmid

    #179230

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p23-mNeongreen-DDX21 DAT

    Plasmid

    #179229

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Use

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    G234D, K235A

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. human ETV6 gRNA-4

    Plasmid

    #133404

    Purpose

    human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas-Cas12m-ΔZF

    Plasmid

    #192276

    Purpose

    Encodes MmCas12m ΔZF (H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    Cas12m ΔZF
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    H459A, C552A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas-dCas12m-ΔZF

    Plasmid

    #192277

    Purpose

    pCas-dCas12m-ΔZF (D485A, H549A, C552A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m ΔZF
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D485A, H549A, C552A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCas_4(I-U)/1_2_VB

    Plasmid

    #186445

    Purpose

    Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4/1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Cas4 domain swapped with Cas4 domain from G. sulf…

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKate2-DrCav1 in pT3TS-Dest

    Plasmid

    #194293

    Purpose

    In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    caveolin (cav1.S Frog)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mKate2-DrCavin1a in pT3TS-Dest

    Plasmid

    #194294

    Purpose

    In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    cavin1a (cavin1a Zebrafish)
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E-HsNRF2 (NFE2L2)

    Plasmid

    #194302

    Purpose

    Multisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRS

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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