We narrowed to 30,944 results for: des.2

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Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-W308Rmut_ABCA3-GFP

Plasmid

#188550

Purpose

Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

ABCA3 (ABCA3 Human)

Use

Lentiviral

Tags

GFP

Mutation

amino acid W308->R308 (ABCA3 gene)

Promoter

EF1aL

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1sgRNA2

Plasmid

#160946

Purpose

Guide RNA 2 to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 gRNA 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lrrc4-AP-His

Plasmid

#71958

Purpose

Expresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4 (Lrrc4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-1102 USP7

Plasmid

#131246

Purpose

Mammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 535-1102. This truncat…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-560 USP7

Plasmid

#131244

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the UBL domains

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Expresses USP7 amino acids 1 - 560. This truncati…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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GABA(A) receptor subunit a2SE

Plasmid

#49169

Purpose

pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

Insert

GABA(A) receptor subunit alpha-2 (GABRA2 Human)

Tags

HA, bovine alpha 1 signal sequence, and pHGFP (pH…

Expression

Mammalian

Mutation

T343A

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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