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We narrowed to 33,667 results for: CaS;

Showing: 1321 - 1340 of 33667 results

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pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1_2_VB

Plasmid

#186442

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1Δ2_VB

Plasmid

#186443

Purpose

Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas_4(I-U)/1_2_VB

Plasmid

#186445

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain swapped with Cas4 domain from G. sulf…

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas12b(RuvC)

Plasmid

#186450

Purpose

Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

dCas12b

Use

CRISPR

Tags

Expression

Mutation

E848A, D977A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_Uricase

Plasmid

#182400

Purpose

Bacterial expression of uricase

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

uricase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS.Sfi c-Akt1

Plasmid

#196376

Purpose

cellular Akt1 in avian expression vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

c-akt1

Use

Retroviral; Avian

Tags

HA

Expression

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

Plasmid

#195495

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

sgRNA (SOX17 Human)

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Usp5

Plasmid

#174873

Purpose

CRISPR vector for generating Usp5 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Usp5 (Usp5 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Wnk1

Plasmid

#174874

Purpose

CRISPR vector for generating Wnk1 STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Wnk1 (Wnk1 Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-1

Plasmid

#184834

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b-gRNA-NT

Plasmid

#184835

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.0

Plasmid

#190795

Purpose

PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

PRE-Hsp70Bb-Cas9-T2A-eGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#189782

Purpose

Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ132-RZ-Cas12j2

Plasmid

#189783

Purpose

Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-RZ-Cas12j2

Plasmid

#173927

Purpose

Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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