We narrowed to 2,420 results for: NIG

1. pLIB_GST-TEV-SRC(WT)

   Plasmid

   #223742

   Purpose

   Expression of recombinant protein for purification, codon optimized for Insect cells

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-CAG-3xNLS-vsfGFP-0

   Plasmid

   #191097

   Purpose

   To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Pantelis Tsoulfas, Murray Blackmore

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-vsfGFP-0
   

   Use

   AAV

   Tags

   Green fluorescent protein bound to enhancer nanob…

   Expression

   Mammalian

   Mutation

   Optimized to Human codon usage

   Promoter

   CMV and CAG

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL452-Sf3b1-K700E

   Plasmid

   #90425

   Purpose

   selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)

   Depositor

   Alex Minella

   Article

   Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

   Insert

   Sf3b1 - left and right homology arms (Sf3b1 Mouse)
   

   Use

   Homology-directed repair vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIB_GST-TEV-SRC (Y530F)

   Plasmid

   #223743

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Insect

   Mutation

   Y530F constitutively active mutant

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-VQR-P2A-GFP-PGK-Puro

   Plasmid

   #110861

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQR
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D1135V, R1335Q, T1337R

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-PARP1-E988K

   Plasmid

   #211580

   Purpose

   CMV driven PARP1-E988K -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains E988K mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Duet1_6xHis-TEV-eGFP-P62

   Plasmid

   #190929

   Purpose

   Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   P62 (SQSTM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2 8xSTAR-mNeonGreen. PGK-puro

   Plasmid

   #136255

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_GST-TEV-EGFP-ATG13

   Plasmid

   #223797

   Purpose

   Expression of recombinant protein for purification

   Depositor

   James Hurley, Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Tags

   GST-TEV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-H2BmGreenLantern

    Plasmid

    #177332

    Purpose

    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

    Depositor

    Pantelis Tsoulfas, Gregory Petsko, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B (Hist1h2bq Rat)
    

    Use

    AAV

    Tags

    mGreenLantern

    Expression

    Mammalian

    Mutation

    This fusion protein was optimized to the Human co…

    Promoter

    CAG

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-IRES-blast GFP-SPNS1

    Plasmid

    #198571

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB1.HMBP.PrS.dEZH2

    Plasmid

    #220241

    Purpose

    Expression of an EZH2 catalytic site mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    dEZH2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    Y731F

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1.HMBP.PrS.JARID2

    Plasmid

    #125166

    Purpose

    Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    JARID2 (JARID2 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-xBE4Gam-P2A-Puro

    Plasmid

    #112676

    Purpose

    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)-BE4Gam
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

    Plasmid

    #118274

    Purpose

    Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Inserts

    bPAC(WT)
    SthK
    

    Use

    AAV

    Tags

    His tag, P2A, mCherry, and myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CamK II

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIV-EGFP.Flag-EZH2-mt2*

    Plasmid

    #220246

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2* (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB1.HMBP.PrS.EPOP

    Plasmid

    #125170

    Purpose

    Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    EPOP (EPOP Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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