We narrowed to 26,352 results for: Nes

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LAMTOR1-APEX2

Plasmid

#215556

Purpose

Stable, inducible expression in lysosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

LAMTOR1-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Cyto-ABKAR

Plasmid

#61510

Purpose

Expression of AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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Endo-APEX2

Plasmid

#215553

Purpose

Stable, inducible expression in endosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-2xFYVE-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pQP-2460

Plasmid

#138973

Purpose

Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q, N496F)

Plasmid

#170139

Purpose

AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-ADAR2-DD(E488Q, N496F)

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-N

Plasmid

#170153

Purpose

AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-RESCUE-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM16467

Plasmid

#105403

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHis-NES(PKI)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCL1023

Plasmid

#252434

Purpose

ASF1A tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

ASF1A (ASF1A Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCL1013

Plasmid

#252433

Purpose

HAT1 tagged on the N-terminus with mCherry and a Nuclear Export Sequence to make it cytoplasmic.

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

HAT1 (HAT1 Human)

Tags

mCherry-NES

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pQP-2363

Plasmid

#138975

Purpose

Relocalization of genomically expressed protein of interest from cytoplasm to nucleus upon near-infrared illumination

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1- iB(GFP) -IRES2-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM16418

Plasmid

#105404

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHis-NES(Rev)-GFP

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM16429

Plasmid

#105405

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHis-NES(PKI)-GFP

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAGM16456

Plasmid

#105402

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHis-NES(Rev)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Golden GATEway Cloning Kit

Plasmid Kit

#1000000054

Purpose

A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.

Depositor

Joachim Wittbrodt

Application

Cloning and Synthetic Biology

Vector Type

Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression

Cloning Type

Golden Gate

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
lenti dCAS-VP64_Blast (Lentiviral Prep)

Viral Prep

#61425-LV

Purpose

Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only
dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

Viral Prep

#61422-LVC

Purpose

Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Promoter

EF1a

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only
CARMEN

Plasmid

#240464

Purpose

Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER

Depositor

Roland Malli

Article

Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111

Insert

CARMEN

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDN80

Plasmid

#61349

Purpose

LINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Type

Empty backbone

Tags

AsLOV2 wt and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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PM-APEX2

Plasmid

#215557

Purpose

Stable, inducible expression in plasma membrane

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Lyn11-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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