We narrowed to 2,724 results for: gem
-
-
pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168170PurposeExpression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
TagsYFPExpressionWormMutationE497K and C566DAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-R244Q
Plasmid#109023Purposestable lentiviral expression of cDNADepositorInsertATL2-R244Q (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationArg244Gln mutation results in dimerization defectPromoterEIF-1a promoterAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(D323A/D325A)
Plasmid#86630PurposeExpresses eGFP-tagged human Akt1(D323A/D325A) mutantDepositorInsertAkt1(D323A/D325A) (AKT1 Human)
ExpressionMammalianAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA010
Plasmid#22843DepositorInsertA DNA containing GFP
UseCotton expression vectorExpressionBacterialMutationCotton Leaf Crumple Virus A component with Coat P…Available SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(T308A/S473A)
Plasmid#86578PurposeExpresses human full-length Akt1(T308A/S473A) phosphomutantDepositorAvailable SinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable SinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PH1(K14A)
Plasmid#86600PurposeExpresses mCherry-tagged PH(K14A) domain of human Akt1DepositorInsertK14A mutant of Akt1 PH domain (AKT1 Human)
ExpressionMammalianAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL3-2
Plasmid#109010PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-HA-UFL1-RAMP4
Plasmid#139863PurposeLentiviral expression of fusion of human UFL1 (full length) with RAMP4DepositorInsertUFL1-RAMP4 (UFL1 Human)
UseLentiviralTagsHAMutationrecombinant fusion of UFL1 (full length) with RAM…Available SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD
Plasmid#113954Purposemammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-568PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCit-Snap25b D179K A199R
Plasmid#53259PurposeSNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.DepositorInsertSNAP-25B (Snap25 Rat)
Tags4aa-linker and mCitrineExpressionMammalianMutationD179K and A199RPromoterCMVAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJRTCLCrVA009
Plasmid#22842DepositorInsertA DNA containing CHLI fragment
UseCotton vigs vectorExpressionBacterialMutationCotton Leaf Crumple Virus Coat Protein (AR1 gene)…Available SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTet02
Plasmid#78284PurposemRFP1 expressed behind an ATc-inducible constructDepositorInsertTet inducible mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL3-5
Plasmid#109011PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTol1-U6abc_tnnt2a_cmlc2-nmKate
Plasmid#238309PurposeDrives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.DepositorInsertnuclear mKate/3 gRNAs targeting tnnt2a
Promotercmlc2 (nmKate); U6 (gRNAs)Available SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mVenus-rRim1[20-227]-mVenus(L68V)
Plasmid#246280PurposeRab10 sensor acceptorDepositorAvailable SinceOct. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)
Plasmid#225438PurposeTranscriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.DepositorInsertCBS-FRT-OCS-BeYDVRepRepA-tNos
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
