We narrowed to 2,567 results for: gem

1. pLIB-GST-3C-CDK1

   Plasmid

   #177011

   Purpose

   Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CDK1 (CDK1 Human)
   

   Tags

   GST-3C

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-HLA-Flag-natT1R3

   Plasmid

   #113950

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Tags

   FLAG and Signal/leader sequence from HLA class I …

   Expression

   Mammalian

   Mutation

   residues 21-852

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetOn-CLIMP63-mCherry-eGFP

   Plasmid

   #139871

   Purpose

   Dox-inducible lentiviral expression of human CLIMP63

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   CLIMP63 (CKAP4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2R3a-TagRFP-OcsT

   Plasmid

   #71270

   Purpose

   Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   TagRFP
   

   Use

   Gateway

   Tags

   4xGly linker and octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV EF1a DIO iChloC 2A dsRed

   Plasmid

   #70762

   Purpose

   An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   EF1a

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Il12b 400bp pro-Luc

   Plasmid

   #20020

   Depositor

   Stephen Smale

   Article

   Plevy et al Mol Cell Biol. 1997 Aug . 17(8):4572-88.

   Insert

   Il12b promoter (Il12b Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. hSyn-BiPOLES-mCerulean

   Plasmid

   #154944

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   BiPOLES
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-X1-Neo-DDRGK1-WT-HA

   Plasmid

   #139845

   Purpose

   Lentiviral expression of human DDRGK1-WT-HA

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   DDRGK1 (DDRGK1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. hMLKL NB(1-182)-2xFv-Venus

    Plasmid

    #106077

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL NB(1-182)-2xFv-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    amino acids 1-182

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBD-Akt13C(S473D)

    Plasmid

    #86577

    Purpose

    Expresses cleavable human full-length Akt1(S473D) phosphomimetic

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130; S473D phos…

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23_Promoter_1-3

    Plasmid

    #210629

    Purpose

    Luciferase experiment for TAL1 promoter 1-3

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 1-3 (TAL1 Human)
    

    Expression

    Mammalian

    Promoter

    Minimal Promoter

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23_Promoter_4

    Plasmid

    #210630

    Purpose

    Luciferase experiment for TAL1 promoter 4

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    promoter 4 (TAL1 Human)
    

    Expression

    Mammalian

    Promoter

    Minimal Promoter

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23_exon 4

    Plasmid

    #210631

    Purpose

    Luciferase experiment for TAL1 exon 4

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1 exon 4 (TAL1 Human)
    

    Expression

    Mammalian

    Promoter

    Minimal Promoter

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. ESR1-luciferase

    Plasmid

    #113351

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    ESR1-enhancer (ESR1 Human)
    

    Use

    Luciferase

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

    Plasmid

    #35500

    Purpose

    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-HA-Flag-natT1R2 FS1

    Plasmid

    #113955

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    delete N-terminal 21 residues, one base pair dele…

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. J23104-mRFP1-331Bb

    Plasmid

    #78274

    Purpose

    mRFP1 behind constitutive promoter J23104 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23104-mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

    Plasmid

    #118278

    Purpose

    Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

    Depositor

    Dietmar Schmitz

    Article

    Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

    Insert

    bPAC(WT)
    

    Use

    AAV

    Tags

    mCherry and myc

    Promoter

    CamKII

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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