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Plasmid#206278PurposeENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.DepositorInsertaeBlue, VSV-G
UseMultimate/gateway entr 3TagsExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S
Plasmid#147461PurposeMammalian Expression of HsSmg7-G100E-siRNAres-shRNAresDepositorInsertHsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)
UseTagsExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S
Plasmid#147462PurposeMammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAresDepositorInsertHsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)
UseTagsExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7-T103E-siRNAres_S
Plasmid#147464PurposeMammalian Expression of HsSmg7-T103E-siRNAres-shRNAresDepositorInsertHsSmg7-T103E-siRNAres-shRNAres (SMG7 Human)
UseTagsExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G an…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S
Plasmid#147471PurposeMammalian Expression of HsSmg5-Q123E-siRNAresDepositorInsertHsSmg5-Q123E-siRNAres (SMG5 Human)
UseTagsExpressionMammalianMutationthree non silent mutation A254G (K85R), T980C (L3…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW02 AAV-TBG-Cre-sgP53-sgRNA
Plasmid#192162PurposeAAV-TBG-Cre-sgP53-sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K468TAG)-FLAG
Plasmid#182658PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K211TAG)-FLAG
Plasmid#182655PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK211TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(R438TAG)-FLAG
Plasmid#182657PurposeExpresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationR438TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker-3xFLAG KI
Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K468TAG)
Plasmid#182663PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K227R-HA
Plasmid#139859PurposeLentiviral expression of human DDRGK1-K227R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK227RPromoterAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K121R-HA
Plasmid#139852PurposeLentiviral expression of the cDNA indicatedDepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK121RPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K116R-HA
Plasmid#139850PurposeLentiviral expression of human DDRGK1-K116R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK116RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K120R-HA
Plasmid#139851PurposeLentiviral expression of human DDRGK1-K120R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK120RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K124R-HA
Plasmid#139853PurposeLentiviral expression of human DDRGK1-K124R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK124RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K128R-HA
Plasmid#139854PurposeLentiviral expression of human DDRGK1-K128R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK128RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K146R-HA
Plasmid#139855PurposeLentiviral expression of human DDRGK1-K146R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK146RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-DDRGK1-K176R-HA
Plasmid#139856PurposeLentiviral expression of human DDRGK1-K176R-HADepositorInsertDDRGK1 (DDRGK1 Human)
UseLentiviralTagsHAExpressionMutationK176RPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only