We narrowed to 6,231 results for: nls

1. pMT-myl7-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80061

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

   Plasmid

   #134334

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA17_Sga
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

   Plasmid

   #134335

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA18_Sma
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

   Plasmid

   #134336

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA19_Ssim
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

   Plasmid

   #134338

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA18_Sga
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

   Plasmid

   #134339

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA19_Spse
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER20-Flag-NLS-hSPDEF delta1

   Plasmid

   #100874

   Purpose

   Lentiviral expression of human SPDEF truncation

   Depositor

   Noah Shroyer

   Article

   Lo et al Gastroenterology. 2017 Jul;153(1):205-21

   Insert

   SPDEF (SPDEF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   truncated protein 1-248aa

   Promoter

   TRE2

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPL10p::pCGTAG (SPL10p::NLS-GFP)

   Plasmid

   #27410

   Depositor

   David Bartel

   Article

   Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

   Insert

   SPL10 promoter (AT1G27370 Mustard Weed)
   

   Use

   Tags

   Expression

   Mutation

   SPL10 promoter transcriptionally fused to a nucle…

   Promoter

   Available Since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

    Plasmid

    #113437

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK 3XFLAG-MKK7-EE-NLS hygro

    Plasmid

    #87780

    Purpose

    Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK7 (Map2k7 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pBluescript II NLS-MC-EGFP-tdTomato (pBET2)

    Plasmid

    #240411

    Purpose

    This plasmid facilitates the assessment of MMR proficiency in human living cells

    Depositor

    Isao Kuraoka

    Article

    Takedachi et al Mutat Res. 2022 Jan-Jun;824:111779. doi:

    Insert

    NLS-EGFP fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid

    #240546

    Purpose

    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.

    Depositor

    Roman Szczęsny

    Article

    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    enDR3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L52M

    Promoter

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

    Plasmid

    #240547

    Purpose

    Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.

    Depositor

    Roman Szczęsny

    Article

    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    nb-enDR3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S48A, K50A

    Promoter

    Available Since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSE152: pFuGW-hUbCp-3CLpro-SV40NLS

    Plasmid

    #240455

    Purpose

    SARS-CoV-2 Protease 3CLpro expression plasmid

    Depositor

    Lior Nissim

    Article

    Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

    Insert

    3CLpro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hUbCp

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-EFS-MCP-3XBFP-2xNLS

    Plasmid

    #236371

    Purpose

    Overexpression of MCP-3XBFP-2xNLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFP-2xnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-EFS-MCP-3XBFP-NLS

    Plasmid

    #236372

    Purpose

    Overexpression of MCP-3XBFPnls in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    MCP-3XBFPnls
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-EFS-Halo-NLS-tDeg

    Plasmid

    #236370

    Purpose

    Overexpression of Halo-NLS-tDeg in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    Halo-NLS-tDeg
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231345

    Purpose

    Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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