We narrowed to 6,675 results for: proc

1. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVE-eGFP

   Plasmid

   #52581

   Purpose

   Lentiviral transfer vector driving expression of eGFP in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. DV-GFP-Rab38-WT-pDEST53

   Plasmid

   #15669

   Depositor

   William Pavan

   Article

   Loftus et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   Rab38 (Rab38 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wildtype Rab38 sequence

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-hDcp2

   Plasmid

   #72214

   Purpose

   bacterial expression of His-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. sgCtr- LentiCRISPRv2

   Plasmid

   #107402

   Purpose

   Lentiviral expression of Cas9 and a control gRNA

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   control gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209725

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a SnoopTag-mEGFP-SpyTag

   Plasmid

   #72325

   Purpose

   Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-mEGFP-SpyTag
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB-GFP

   Plasmid

   #11155

   Purpose

   Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   Ubiquitin C promoter (UBC Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVP91A-pcaHG

    Plasmid

    #113766

    Purpose

    Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

    Depositor

    John Lipscomb

    Article

    Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

    Inserts

    β subunit of Protocatechuate 3,4- Dioxygenase
    α subunit of Protocatechuate 3,4- Dioxygenase
    

    Use

    Tags

    Met-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T5 promoter

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFZ19

    Plasmid

    #36184

    Depositor

    Daniel Voytas

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2010 Jun 29. 1

    Type

    Empty backbone

    Use

    Plant t-dna vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    XVE

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDawn-Ag43

    Plasmid

    #107742

    Purpose

    Expresses Ag43 under the control of a blue-light transcriptional regulator

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

    Insert

    Ag43 (flu )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    removed all pstI cut sites with silent mutations,…

    Promoter

    pLambda

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYC-C

    Plasmid

    #207114

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

    Plasmid

    #209728

    Purpose

    Expresses cytosolic high affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    Available since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYC-N

    Plasmid

    #207113

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge