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Plasmid#138562PurposeExpresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSDDepositorInsertVRQR
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationD1135V, G1218R, R1335Q, T1337RPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-dSV40-CTLA-4-mGFP
Plasmid#223594PurposeLentiviral expression of human CTLA4 with mGFP tag in mammalian cellsDepositorInsertCTLA4 (CTLA4 Human)
UseLentiviral; mammalian expressionTagsmGFPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-B
Plasmid#104448PurposeExpression of human VAP-B fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein B (VAPB Human)
UseTagsEGFP and HAExpressionMammalianMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pITGA4-GFP
Plasmid#81178PurposeMammalian expression of human full length ITGA4-EGFPDepositorInsertITGA4 (ITGA4 Human)
UseTagsEGFPExpressionMammalianMutationContains KLGFFKR motif; A1002L (please see deposi…PromoterCMVAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TET1-dCas9
Plasmid#235593PurposeTet1CD fused to N-terminus of dCas9DepositorInsertTET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
UseCRISPRTags3xNLS, TET1 CD, and tagBFPExpressionMammalianMutationPromoterEF1aAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
LsgRNA-MS2
Plasmid#235597PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-MVPH
Plasmid#235600PurposeExpresses the MS2-VP64-P65-HSF1 fusion proteinDepositorInsertMS2-VP64-P65-HSF1
UseLentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1aAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0952
Plasmid#143894PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-CUP1pr
Plasmid#221109PurposeCopper-inducible gene expressionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only