257,152 results

1. pLV-tetO-Tfap2c

   Plasmid

   #70269

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   transcription factor AP-2, gamma (Tfap2c Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-fDIO EYFP (AAV8)

   Viral Prep

   #55641-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a

   Available Since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

3. TFORF0096

   Plasmid

   #142459

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pF1-Hs Cdk1/cyclin B1

   Plasmid

   #177330

   Purpose

   Co-expression of the human active Cdk1/cyclin B1 kinase complex

   Depositor

   Andreas Boland

   Article

   Yu et al Nature. 2021 Aug;596(7870):138-142. doi:

   Inserts

   Cyclin-dependent kinase 1 (CDK1 Human)
   G2/mitotic-specific cyclin-B1 (CCNB1 Human)
   

   Tags

   2 x Strep-tag and 8 x His

   Expression

   Insect

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p11d-sctRPA

   Plasmid

   #102614

   Purpose

   Expression of S. cerevisiea RPA in E.coli

   Depositor

   Marc Wold

   Article

   Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

   Inserts

   RPA1 (RFA1 Budding Yeast)
   RPA2 (RFA2 Budding Yeast)
   RPA3 (RFA3 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   RFA2 has intron deleted

   Promoter

   T7

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p11d-sctRPA

   Plasmid

   #102614

   Purpose

   Expression of S. cerevisiea RPA in E.coli

   Depositor

   Marc Wold

   Article

   Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

   Inserts

   RPA1 (RFA1 Budding Yeast)
   RPA2 (RFA2 Budding Yeast)
   RPA3 (RFA3 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   RFA2 has intron deleted

   Promoter

   T7

   Available Since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-EF1afull-H2B-mCherry-P2A-Fluc

   Plasmid

   #207253

   Purpose

   Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   H2B-mCherry-P2A-Fluc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1alpha_full

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0689

   Plasmid

   #141662

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNF4G (HNF4G Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHeACT-mEGFP-NLS

    Plasmid

    #205003

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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