We narrowed to 4,385 results for: 187

1. pCAG-EGFP-C-IFT74

   Plasmid

   #218730

   Purpose

   Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

   Insert

   IFT74 (IFT74 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nrp2.3-AP-His

   Plasmid

   #71974

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.3 (Nrp2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Desmoplakin II-YPet(short)

   Plasmid

   #118714

   Purpose

   The human desmoplakin II is C-terminally tagged with YPet(short) to serve as wild type control.

   Depositor

   Carsten Grashoff

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   Desmoplakin II-YPet(short) (DSP Human)
   

   Use

   Retroviral

   Tags

   YPet(short)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMito-LSSmKate1-N1

   Plasmid

   #31878

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   human cytochrome C oxidase subunit VIII (COX8A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   Available since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nrp2.1-Fc-His

   Plasmid

   #72098

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.1 (Nrp2 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-4mtD3cpv

   Plasmid

   #181874

   Purpose

   Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

   Insert

   4mtD3cpv
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   the 4mtD3cpv CDS differs from the recorded GeneBa…

   Promoter

   CaMKIIa

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDG335

   Plasmid

   #100899

   Purpose

   SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9 Nickase
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A mutant converts to Nickase

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-IFT27

   Plasmid

   #218722

   Purpose

   Expresses N-terminally EGFP-tagged IFT27 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT27 (IFT27 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIR-21 Reporter

   Plasmid

   #71870

   Purpose

   Mammalian expression vector for the analysis of miR-21 activity

   Depositor

   Mien-Chie Hung

   Article

   Shen et al Nature. 2013 May 16;497(7449):383-7. doi

   Insert

   Reverse complementary sequence of miR-21
   

   Use

   Tags

   firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDG330

    Plasmid

    #100898

    Purpose

    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcmv3-STING-C88S-FLAG

    Plasmid

    #198187

    Purpose

    Mammalian expression of FLAG-tagged mouse STING1 C88S

    Depositor

    Yun Zhao

    Article

    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

    Insert

    TMEM173 (Sting1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-LSSmKate2-N1

    Plasmid

    #31879

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    human cytochrome C oxidase subunit VIII (COX8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid

    #114187

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and mEOS2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV-SLC7A11-sh926R-FLAG-IRES-Hygro

    Plasmid

    #118702

    Purpose

    lentiviral vector experessing SLC7A11 insensitive to hairpin TRCN0000288926

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    wt , SLC7A11 sh926R

    Promoter

    Available since

    Dec. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-shNCLX-2

    Plasmid

    #181871

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-shNCLX-1

    Plasmid

    #181870

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-V5-PTPN14-del-C

    Plasmid

    #61005

    Purpose

    Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete C (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 934-1187

    Promoter

    CMV

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTER E2F2shRNA human

    Plasmid

    #66884

    Purpose

    Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F2

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F transcription factor 2 (E2F2 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 Tet

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pENTR4 CDS A. thaliana PHOT1

    Plasmid

    #209187

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT3G45780.1 (PHOT1 Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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