We narrowed to 3,581 results for: Gla

1. pYI2294

   Plasmid

   #228352

   Purpose

   Methanol-inducible ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   KpAOX1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYI2292

   Plasmid

   #228350

   Purpose

   Methanol-inducible ALX-0171 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   KpAOX1 promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYI2293

   Plasmid

   #228351

   Purpose

   Constitutive ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   KpGAPDH promoter

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT1461

   Plasmid

   #228319

   Purpose

   Doxycycline-regulatable ALX-0081 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   Doxycycline-regulatable synthetic promoter (KpTc7…

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3_5UTR_Myc_FLuc RBM42 deletion

   Plasmid

   #229503

   Purpose

   Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   Myc 5'UTR_Del 363
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of bp 363-394

   Promoter

   SV40

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3_5UTR_Myc_FLuc Compensatory Mutant

   Plasmid

   #229504

   Purpose

   Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   Myc 5'UTR_CompMutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutations in bp 363-394

   Promoter

   SV40

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-28b Salmonella NAT_6H C-terminal

   Plasmid

   #223436

   Purpose

   Salmonella NAT expression vector with C-terminal His6 tag

   Depositor

   Emily Balskus

   Article

   Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

   Insert

   Salmonella NAT
   

   Use

   Tags

   His6

   Expression

   Mutation

   Promoter

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBpuro-miRFP703-eDHFR(69K6)-cRaf

   Plasmid

   #184717

   Purpose

   iRaf

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

   Insert

   miRFP703-eDHFR(69K6)-cRaf (RAF1 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

   Plasmid

   #191631

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   Dopamine dehydroxylase (R506S mutation, inactive form)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

    Plasmid

    #191651

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-DEST42 -SSRP1

    Plasmid

    #170439

    Purpose

    Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

    Depositor

    Joan Conaway

    Article

    Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

    Insert

    ssrp1 (Ssrp Fly)
    

    Use

    Tags

    6xHis and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDECKO_mCherry_NFE2

    Plasmid

    #140105

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDECKO_mCherry_TREML2

    Plasmid

    #140106

    Purpose

    CRISPR-Cas9 Knock down

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDECKO_mCherry_LINC02432

    Plasmid

    #140107

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDECKO_mCherry_MIR3945HG

    Plasmid

    #140108

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDECKO_mCherry_Intergenic

    Plasmid

    #140100

    Purpose

    CRISPR-Cas9 library validation (negative control)

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO_mCherry_FURIN

    Plasmid

    #140103

    Purpose

    CRISPR-Cas9 library validation

    Depositor

    Roderic Guigo

    Article

    Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    gRNA1 under U6 and gRNA2 under H1

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SMR3B-bio-His

    Plasmid

    #52183

    Purpose

    Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SMR3B (SMR3B Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

    Plasmid

    #157623

    Purpose

    Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL5 (KIR2DL5A Human)
    

    Use

    Tags

    6xHis and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

    Plasmid

    #199390

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi-SUMOEu

    Expression

    Bacterial

    Mutation

    Promoter

    T5-LacO

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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