We narrowed to 59,403 results for: cloning

1. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2R3z-BASI-ccdB-BSAI

   Plasmid

   #118389

   Purpose

   Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   ccdB
   

   Use

   Unspecified

   Mutation

   See Depositor Comments Section

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSS9

   Plasmid

   #71655

   Purpose

   Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   gfpuv
   

   Use

   Synthetic Biology

   Promoter

   T7A1

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6Z-806

   Plasmid

   #85255

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

   Plasmid

   #162949

   Purpose

   Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

   Plasmid

   #162918

   Purpose

   Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   firefly luciferase/green fluorescent protein fusion with upstream IRES sequence
   

   Use

   Synthetic Biology

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6Z-101

   Plasmid

   #85226

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221_zCas9

   Plasmid

   #213913

   Purpose

   Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Sébastien Mongrand

   Article

   Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi

   Insert

   zCas9
   

   Use

   CRISPR

   Mutation

   Zea mays codon-optimized Cas9

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. C513-E06: attB5r-pA-polyhedrinp>-attB1r

   Plasmid

   #162948

   Purpose

   Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   AcMNPV polyhedrin promoter with upstream polyA sequence
   

   Use

   Synthetic Biology

   Available Since

   April 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

    Plasmid

    #162965

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Halotag fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

    Plasmid

    #162964

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

    Plasmid

    #162957

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

    Plasmid

    #162955

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

    Plasmid

    #162954

    Purpose

    Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
    

    Use

    Synthetic Biology

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH6Z-707

    Plasmid

    #85249

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pH6Z-709

    Plasmid

    #85251

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pH6Z-710

    Plasmid

    #85252

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pH6Z-805

    Plasmid

    #85254

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pH6Z-711

    Plasmid

    #85253

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH6Z-807

    Plasmid

    #85256

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge