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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CRE recombinase

Plasmid

#62730

Purpose

Expresses Cre recombinase protein in bacterial cells

Depositor

Niels Geijsen

Publication

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

Cre recombinase

Use

Cre/Lox

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Publication

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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S. cerevisiae Advanced Gateway Destination Vectors

Plasmid kit

#1000000011

Purpose

Destination vectors for tagging and expression of genes in S. cerevisiae.

Depositor

Susan Lindquist

Application

Cloning and Synthetic Biology, Gene Expression and Labeling

Vector type

Yeast Expression

Cloning type

Gateway

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLenti.PGK.blast-Renilla_Luciferase

Plasmid

#74444

Purpose

Control lentiviral vector expressing Renilla luciferase

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Renilla Luciferase

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pLenti.PGK.blast-Renilla_Luciferase

Plasmid

#74444

Purpose

Control lentiviral vector expressing Renilla luciferase

Depositor

Reuben Shaw

Publication

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Renilla Luciferase

Use

Lentiviral

Expression

Mammalian

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCR4-24XPP7SL

Plasmid

#31864

Depositor

Robert Singer

Publication

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7 Stem Loop Cassette

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCR4-24XPP7SL

Plasmid

#31864

Depositor

Robert Singer

Publication

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

24xPP7 Stem Loop Cassette

Available since

Aug. 31, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Publication

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Publication

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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gRNA_GFP-T2

Plasmid

#41820

Purpose

Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_GFP-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pTight-9-124-BclxL

Plasmid

#60857

Purpose

2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pTight-9-124-BclxL

Plasmid

#60857

Purpose

2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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Flag-HA-USP10

Plasmid

#22543

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP10 (USP10 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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