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Plasmid#121806PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETaDepositorInsertHA epitope tag-polyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]
Plasmid#153532PurposeAAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertSomArchon-GFP
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationPromoterEF1α1.1Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
Plasmid#69586PurposeExpresses mutated p53-L344P tagged with mKate2 and split N-term mVenusDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - N termExpressionMammalianMutationp53 L344P mutation that prevents dimerization and…PromoterEF1alphaAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH
Plasmid#235276PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2(miR.FF4)-bGH
Plasmid#235285PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH
Plasmid#235294PurposeComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235270PurposeComMAND open-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235271PurposeComMAND closed-loop circuit regulating mRuby2, EF1aDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2(miRE.FF4)-bGH
Plasmid#235289PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH
Plasmid#235283PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF4DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miRE.FF5)-bGH
Plasmid#235262PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2(miRE.FF4)-bGH
Plasmid#235288PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-miRE.FF4-bGH
Plasmid#235290PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH
Plasmid#235261PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2-miR.FF4-bGH
Plasmid#235287PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2-miRE.FF4-bGH
Plasmid#235291PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF5x1-mRuby2(miRE.FF5)-bGH
Plasmid#235263PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-miR.FF4-bGH
Plasmid#235286PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x2-bGH
Plasmid#235292PurposeComMAND open-loop circuit regulating mRuby2 with 2 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-EYFP
Plasmid#231926PurposeCre-on/Flp-on EYFP under the Ef1a promoterDepositorInserteYFP with introns
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only